ArticleBioMed research international2013
HTR8/SVneo cells display trophoblast progenitor cell-like characteristics indicative of self-renewal, repopulation activity, and expression of "stemness-" associated transcription factors.
Article in BioMed research international, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 65 citations in OpenAlex.
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- The Landscape of Disulfidptosis in Preeclampsia Reveals a Novel 5-Gene Diagnostic Signature via Machine Learning.International journal of women's health · 2026Article
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- The modeling of human implantation and early placentation: achievements and perspectives.Human reproduction update · 2025Review
- Using transcriptomic signatures to elucidate individual and mixture effects of inorganic arsenic and manganese in human placental trophoblast HTR-8/SVneo cells.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Modeling human placental biology: a review of organoid technologies.Frontiers in cell and developmental biology · 2025Review
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- TNF-α-Induced Downregulation of ID2 in Human First Trimester Trophoblast Cells.Asian journal of biological sciences · 2025Article
- Transcriptomic comparison of in vitro models of the human placenta.bioRxiv : the preprint server for biology · 2024Article
- Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development.Cell and tissue research · 2022Article
- Origination of LTR Retroelement-Derived NYNRIN Coincides with Therian Placental Emergence.Molecular biology and evolution · 2022Article
- Low-Dose Tacrolimus Promotes the Migration and Invasion and Nitric Oxide Production in the Human-Derived First Trimester Extravillous Trophoblast Cells In Vitro.International journal of molecular sciences · 2022Article
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- Extravillous trophoblast migration and invasion: Impact of environmental chemicals and pharmaceuticals.Reproductive toxicology (Elmsford, N.Y.) · 2022Review
- Targeting Aberrantly Elevated Sialyl Lewis A as a Potential Therapy for Impaired Endometrial Selection Ability in Unexplained Recurrent Miscarriage.Frontiers in immunology · 2022Article
- Role of microRNAs in trophoblast invasion and spiral artery remodeling: Implications for preeclampsia.Frontiers in cell and developmental biology · 2022Review
- Increased cell-free fetal DNA release after apoptosis and sterile inflammation in human trophoblast cells.American journal of reproductive immunology (New York, N.Y. : 1989) · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionJEG3 is a choriocarcinoma--and HTR8/SVneo a transformed extravillous trophoblast--cell line often used to model the physiologically invasive extravillous trophoblast. Past studies suggest that these cell lines possess some stem or progenitor cell characteristics. Aim was to study whether these cells fulfill minimum criteria used to identify stem-like (progenitor) cells. In summary, we found that the expression profile of HTR8/SVneo (CDX2+, NOTCH1+, SOX2+, NANOG+, and OCT-) is distinct from JEG3 (CDX2+ and NOTCH1+) as seen only in human-serum blocked immunocytochemistry. This correlates with HTR8/SVneo's self-renewal capacities, as made visible via spheroid formation and multi-passagability in hanging drops protocols paralleling those used to maintain embryoid bodies. JEG3 displayed only low propensity to form and reform spheroids. HTR8/SVneo spheroids migrated to cover and seemingly repopulate human chorionic villi during confrontation cultures with placental explants in hanging drops. We conclude that HTR8/SVneo spheroid cells possess progenitor cell traits that are probably attained through corruption of "stemness-" associated transcription factor networks. Furthermore, trophoblastic cells are highly prone to unspecific binding, which is resistant to conventional blocking methods, but which can be alleviated through blockage with human serum.
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