ArticlePloS one2013
Composition of the vaginal microbiota in women of reproductive age--sensitive and specific molecular diagnosis of bacterial vaginosis is possible?
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers, 2 of them syntheses that pooled it.
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Who cites it
114 citing papers in PubMed, 2 syntheses or guidelines pooled it, 229 citations in OpenAlex.
- Contribution ofFrontiers in cellular and infection microbiology · 2021Pooled it
- The vaginal microbiota: what have we learned after a decade of molecular characterization?PloS one · 2014Pooled it
- Sexual practices have a significant impact on the vaginal microbiota of women who have sex with women.Scientific reports · 2019Trial
- Bacterial communities found in placental tissues are associated with severe chorioamnionitis and adverse birth outcomes.PloS one · 2017Trial
- Trial
- Comprehensive Diagnosis of Abnormal Vaginal Discharge Using qPCR-Based Microbial Dysbiosis Indices.Diagnostics (Basel, Switzerland) · 2026Article
- Dynamics of Extracellular Traps in Vaginal Dysbiosis Associated withInternational journal of molecular sciences · 2026Article
- The Vaginal Microbiome and Host Health: Implications for Cervical Cancer Progression.International journal of molecular sciences · 2026Review
- Vaginal microbiome and preterm birth: Composition, mechanisms and microbiota‑directed therapies (Review).International journal of molecular medicine · 2025Review
- Article
- Drug and therapeutic intravaginal delivery targeting diseases in the female reproductive tract: A mathematical modeling perspective.Journal of controlled release : official journal of the Controlled Release Society · 2025Review
- MiCoDe: a web tool for performing microbiome community detection using a Bayesian weighted stochastic block model.Bioinformatics (Oxford, England) · 2025Article
- Dual probiotic and antibiotic therapy targeting bacterial vaginosis: an integrated experimental/computational modeling perspective.Biomedical engineering advances · 2025Article
- The Role of the Genital Tract Microbiome in Human Fertility: A Literature Review.Journal of clinical medicine · 2025Review
- Influences of gestational diabetes mellitus on the changes in the vaginal microbiota from antepartum to postpartum.BMC pregnancy and childbirth · 2025Article
- A Generalized Bayesian Stochastic Block Model for Microbiome Community Detection.Statistics in medicine · 2025Article
- Interaction of vaginal microbiota and biomarkers in Premature rupture of membranes: from bench to beside.Frontiers in immunology · 2025Review
- Perinatal factors influencing the earliest establishment of the infant microbiome.Microbiome research reports · 2025Article
- Evaluation of Metronidazole Resistance of Vaginal Swab Isolates from South African Women Treated for Bacterial Vaginosis.Antibiotics (Basel, Switzerland) · 2024Article
- Sustained dual delivery of metronidazole and viable Lactobacillus crispatus from 3D-printed silicone shells.Biomaterials advances · 2024Article
54 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectiveBacterial vaginosis (BV) is the most common vaginal disorder, characterized by depletion of the normal lactobacillus-dominant microbiota and overgrowth of commensal anaerobic bacteria. This study aimed to investigate the composition of the vaginal microbiota in women of reproductive age (healthy women and women with BV), with the view of developing molecular criteria for BV diagnosis. MATERIALS AND
methodsVaginal samples from 163 women (79 control, 73 BV and 11 intermediate (Lactobacillary grade II flora) cases) were analyzed using 454 pyrosequencing of the hypervariable regions V3-V4 of the 16S rRNA gene and 16 quantitative bacterial species/genus-specific real-time PCR assays. Sensitivities and specificities of potential BV markers were computed using the Amsel criteria as reference standard for BV. The use of quantitative thresholds for prediction of BV, determined for both relative abundance measured with 454 pyrosequencing and bacterial load measured with qPCR, was evaluated.
resultsRelative to the healthy women, the BV patients had in their vaginal microbiota significantly higher prevalence, loads and relative abundances of the majority of BV associated bacteria. However, only Gardnerella vaginalis, Atopobium vaginae, Eggerthella, Prevotella, BVAB2 and Megasphaera type 1 detected at or above optimal thresholds were highly predictable for BV, with the best diagnostic accuracy shown for A. vaginae. The depletion of Lactobacillus species combined with the presence of either G. vaginalis or A. vaginae at diagnostic levels was a highly accurate BV predictor.
conclusionsQuantitative determination of the presence of G. vaginalis, A. vaginae, Eggerthella, Prevotella, BVAB2 and Megasphaera type 1 as well as the depletion of Lactobacillus was highly accurate for BV diagnosis. Measurements of abundance of normal and BV microbiota relative to total bacteria in vaginal fluid may provide more accurate BV diagnosis, and be used for test-of-cure, rather than qualitative detection or absolute counts of BV related microorganisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.