ArticleJournal of bacteriology2013
Structural requirement in Clostridium perfringens collagenase mRNA 5' leader sequence for translational induction through small RNA-mRNA base pairing.
Article in Journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Genome MLST scheme for tracing genetic diversity and multidrug resistance of food animal-derivedCurrent research in food science · 2025Article
- Review
- Hfq protein and GcvB small RNA tailoring ofRNA biology · 2023Article
- Protein Truncating Variants ofFrontiers in cellular and infection microbiology · 2021Article
- Temperature-regulated heterogeneous extracellular matrix gene expression defines biofilm morphology in Clostridium perfringens.NPJ biofilms and microbiomes · 2020Article
- An update on the human and animal enteric pathogen Clostridium perfringens.Emerging microbes & infections · 2018Review
- Immunoactive Clostridial Membrane Vesicle Production Is Regulated by a Sporulation Factor.Infection and immunity · 2017Article
- sRNA-mediated activation of gene expression by inhibition of 5'-3' exonucleolytic mRNA degradation.eLife · 2017Article
- Rethinking the role of alpha toxin in Clostridium perfringens-associated enteric diseases: a review on bovine necro-haemorrhagic enteritis.Veterinary research · 2017Review
- Role of RNase Y in Clostridium perfringens mRNA Decay and Processing.Journal of bacteriology · 2017Article
- The Mechanisms of Virulence Regulation by Small Noncoding RNAs in Low GC Gram-Positive Pathogens.International journal of molecular sciences · 2015Review
- Target activation by regulatory RNAs in bacteria.FEMS microbiology reviews · 2015Review
- A sporulation factor is involved in the morphological change of Clostridium perfringens biofilms in response to temperature.Journal of bacteriology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Gram-positive anaerobic bacterium Clostridium perfringens is pathogenic to humans and animals, and the production of its toxins is strictly regulated during the exponential phase. We recently found that the 5' leader sequence of the colA transcript encoding collagenase, which is a major toxin of this organism, is processed and stabilized in the presence of the small RNA VR-RNA. The primary colA 5'-untranslated region (5'UTR) forms a long stem-loop structure containing an internal bulge and masks its own ribosomal binding site. Here we found that VR-RNA directly regulates colA expression through base pairing with colA mRNA in vivo. However, when the internal bulge structure was closed by point mutations in colA mRNA, translation ceased despite the presence of VR-RNA. In addition, a mutation disrupting the colA stem-loop structure induced mRNA processing and ColA-FLAG translational activation in the absence of VR-RNA, indicating that the stem-loop and internal bulge structure of the colA 5' leader sequence is important for regulation by VR-RNA. On the other hand, processing was required for maximal ColA expression but was not essential for VR-RNA-dependent colA regulation. Finally, colA processing and translational activation were induced at a high temperature without VR-RNA. These results suggest that inhibition of the colA 5' leader structure through base pairing is the primary role of VR-RNA in colA regulation and that the colA 5' leader structure is a possible thermosensor.
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