ArticleExperimental physiology2013
Resolution of hyposmotic stress in isolated mouse ventricular myocytes causes sealing of t-tubules.
Article in Experimental physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- A new approach to the determination of tubular membrane capacitance: passive membrane electrical properties under reduced electrical conductivity of the extracellular solution.Pflugers Archiv : European journal of physiology · 2022Article
- Fraction of the T-Tubular Membrane as an Important Parameter in Cardiac Cellular Electrophysiology: A New Way of Estimation.Frontiers in physiology · 2022Article
- Nano-scale morphology of cardiomyocyte t-tubule/sarcoplasmic reticulum junctions revealed by ultra-rapid high-pressure freezing and electron tomography.Journal of molecular and cellular cardiology · 2021Article
- The mechanism of osmotically induced sealing of cardiac t tubules.American journal of physiology. Heart and circulatory physiology · 2020Article
- Membrane curvature sensing of the lipid-anchored K-Ras small GTPase.Life science alliance · 2019Article
- Diffusional and Electrical Properties of T-Tubules Are Governed by Their Constrictions and Dilations.Biophysical journal · 2018Article
- Cholesterol Protects Against Acute Stress-Induced T-Tubule Remodeling in Mouse Ventricular Myocytes.Frontiers in physiology · 2018Article
- Small membrane permeable molecules protect against osmotically induced sealing of t-tubules in mouse ventricular myocytes.American journal of physiology. Heart and circulatory physiology · 2016Article
- Free Fatty Acid Effects on the Atrial Myocardium: Membrane Ionic Currents Are Remodeled by the Disruption of T-Tubular Architecture.PloS one · 2015Article
- Rapid actin-cytoskeleton-dependent recruitment of plasma membrane-derived dysferlin at wounds is critical for muscle membrane repair.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Ca(2+) homeostasis in sealed t-tubules of mouse ventricular myocytes.Journal of molecular and cellular cardiology · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
It has recently been shown that various stress-inducing manipulations in isolated ventricular myocytes may lead to significant remodelling of t-tubules. Osmotic stress is one of the most common complications in various experimental and clinical settings. This study was therefore designed to determine the effects of a physiologically relevant type of osmotic stress, hyposmotic challenge, to the integrity of the t-tubular system in mouse ventricular myocytes using the following two approaches: (1) electrophysiological measurements of membrane capacitance and inward rectifier (IK1) tail currents originating from K(+) accumulation in t-tubules; and (2) confocal microscopy of fluorescent dextrans trapped in sealed t-tubules. Importantly, we found that removal of '0.6 Na' (60% NaCl) hyposmotic solution, but not its application to myocytes, led to a ∼27% reduction in membrane capacitance, a ∼2.5-fold reduction in the amplitude of the IK1 tail current and a ∼2-fold reduction in the so-called IK1 'inactivation' (due to depletion of t-tubular K(+)) at negative membrane potentials; all these data were consistent with significant detubulation. Confocal imaging experiments also demonstrated that extracellularly applied dextrans become trapped in sealed t-tubules only upon removal of hyposmotic solutions, i.e. during the shrinking phase, but not during the initial swelling period. In light of these data, relevant previous studies, including those on excitation-contraction coupling phenomena during hyposmotic stress, may need to be reinterpreted, and the experimental design of future experiments should take into account the novel findings.
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