ArticleApplied and environmental microbiology2013
Functional screening of a metagenomic library reveals operons responsible for enhanced intestinal colonization by gut commensal microbes.
Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Barcoded overexpression screens in gut Bacteroidales identify genes with roles in carbon utilization and stress resistance.Nature communications · 2024Article
- An Insight into Functional Metagenomics: A High-Throughput Approach to Decipher Food-Microbiota-Host Interactions in the Human Gut.International journal of molecular sciences · 2023Review
- New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota.Communications biology · 2023Article
- Role of metagenomics in prospecting novel endoglucanases, accentuating functional metagenomics approach in second-generation biofuel production: a review.Biomass conversion and biorefinery · 2023Review
- Exploring Bacterial Attributes That Underpin Symbiont Life in the Monogastric Gut.Applied and environmental microbiology · 2022Review
- An efficient system for intestinal on-site butyrate production using novel microbiome-derived esterases.Journal of biological engineering · 2021Article
- From homeostasis to pathology: decrypting microbe-host symbiotic signals in the intestinal crypt.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- A single gene of a commensal microbe affects host susceptibility to enteric infection.Nature communications · 2016Article
- Public health evolutionary biology of antimicrobial resistance: priorities for intervention.Evolutionary applications · 2015Article
- Protective role of gut commensal microbes against intestinal infections.Journal of microbiology (Seoul, Korea) · 2014Review
- The yin and yang of bacterial resilience in the human gut microbiota.Journal of molecular biology · 2014Review
- Metagenomics and novel gene discovery: promise and potential for novel therapeutics.Virulence · 2014Review
- Combined metagenomic and phenomic approaches identify a novel salt tolerance gene from the human gut microbiome.Frontiers in microbiology · 2014Article
- Coupled high-throughput functional screening and next generation sequencing for identification of plant polymer decomposing enzymes in metagenomic libraries.Frontiers in microbiology · 2013Article
- Developing amSystemsArticle
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evidence suggests that gut microbes colonize the mammalian intestine through propagation as an adhesive microbial community. A bacterial artificial chromosome (BAC) library of murine bowel microbiota DNA in the surrogate host Escherichia coli DH10B was screened for enhanced adherence capability. Two out of 5,472 DH10B clones, 10G6 and 25G1, exhibited enhanced capabilities to adhere to inanimate surfaces in functional screens. DNA segments inserted into the 10G6 and 25G1 clones were 52 and 41 kb and included 47 and 41 protein-coding open reading frames (ORFs), respectively. DNA sequence alignments, tetranucleotide frequency, and codon usage analysis strongly suggest that these two DNA fragments are derived from species belonging to the genus Bacteroides. Consistent with this finding, a large portion of the predicted gene products were highly homologous to those of Bacteroides spp. Transposon mutagenesis and subsequent experiments that involved heterologous expression identified two operons associated with enhanced adherence. E. coli strains transformed with the 10a or 25b operon adhered to the surface of intestinal epithelium and colonized the mouse intestine more vigorously than did the control strain. This study has revealed the genetic determinants of unknown commensals (probably resembling Bacteroides species) that enhance the ability of the bacteria to colonize the murine bowel.
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