Evidence map›Paper›PMID 23584769›Full record

ArticleApplied and environmental microbiology2013

Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.

F Heath Damron, Elizabeth S McKenney, Mariette Barbier, George W Liechti, Herbert P Schweizer, Joanna B Goldberg

Open access · bronzeAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
4.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 63 citations in OpenAlex.

  1. Article
  2. Article
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  4. Low-Irradiance Antimicrobial Blue Light-Bathing Therapy for Wound Infection Control.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Antibiotics (Basel, Switzerland) · 2023
    Article
  11. Efficacy of aInfection and immunity · 2023
    Article
  12. Article
  13. Application of the LuminescentFoods (Basel, Switzerland) · 2023
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

F Heath DamronDepartment of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, VA, USA.
Elizabeth S McKenney
Mariette Barbier
George W Liechti
Herbert P Schweizer
Joanna B Goldberg
University of Virginia Health System · USChildren's Healthcare of Atlanta · USColorado State University · US

Funding

Vascular stabilization as a broad theraprutic platform for biodefenseU54AI065357 · NIAID · COLORADO STATE UNIVERSITY-FORT COLLINS · PI BELISLE, JOHN T · 2005 to 2013
$77.5M
Cancer Research Training Program: From Molecular Mechanisms to Therapeutic StrategiesT32CA009109 · NCI · UNIVERSITY OF VIRGINIA CHARLOTTESVILLE · PI Andrew Carl Dudley, Melanie R Rutkowski · 1985 to 2026
$13.9M
Live Attenuated Recombinant Bacterial Delivery of Polysaccharide Vaccine AntigensR01AI068112 · NIAID · UNIVERSITY OF VIRGINIA · PI GOLDBERG, JOANNA B · 2006 to 2010
$2.0M
NCI NIH HHS 5T32 CA 009109NCI NIH HHS T32 CA009109NIAID NIH HHS R01 AI068112NIAID NIH HHS U54 AI065357
6 · The paper itself

Abstract

We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

Indexed as

BiomarkersChromosome MappingDNA PrimersGene Expression ProfilingGenes, ReporterGenetic VectorsGram-Negative BacteriaLuciferases, BacterialPlasmidsPseudomonas aeruginosaTrimethoprim ResistanceBiomarkersDNA PrimersLuciferases, Bacterial

Identifiers

PMID23584769
PMCPMC3697578
OpenAlexW2147164329

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.