ArticleRNA (New York, N.Y.)2013
Identification of purple sea urchin telomerase RNA using a next-generation sequencing based approach.
Article in RNA (New York, N.Y.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Biogenesis of telomerase RNA from a protein-coding mRNA precursor.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Monophyletic Origin and Divergent Evolution of Animal Telomerase RNA.Molecular biology and evolution · 2021Article
- Article
- Evolutionary perspectives of telomerase RNA structure and function.RNA biology · 2016Review
- An updated human snoRNAome.Nucleic acids research · 2016Article
- Structure and function of echinoderm telomerase RNA.RNA (New York, N.Y.) · 2016Article
- Diverse mechanisms for spliceosome-mediated 3' end processing of telomerase RNA.Nature communications · 2015Article
- A self-regulating template in human telomerase.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Progress in structural studies of telomerase.Current opinion in structural biology · 2014Review
- The transcriptome of the NZ endemic sea urchin Kina (Evechinus chloroticus).BMC genomics · 2014Article
- RNA connectivity requirements between conserved elements in the core of the yeast telomerase RNP.The EMBO journal · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomerase is a ribonucleoprotein (RNP) enzyme essential for telomere maintenance and chromosome stability. While the catalytic telomerase reverse transcriptase (TERT) protein is well conserved across eukaryotes, telomerase RNA (TR) is extensively divergent in size, sequence, and structure. This diversity prohibits TR identification from many important organisms. Here we report a novel approach for TR discovery that combines in vitro TR enrichment from total RNA, next-generation sequencing, and a computational screening pipeline. With this approach, we have successfully identified TR from Strongylocentrotus purpuratus (purple sea urchin) from the phylum Echinodermata. Reconstitution of activity in vitro confirmed that this RNA is an integral component of sea urchin telomerase. Comparative phylogenetic analysis against vertebrate TR sequences revealed that the purple sea urchin TR contains vertebrate-like template-pseudoknot and H/ACA domains. While lacking a vertebrate-like CR4/5 domain, sea urchin TR has a unique central domain critical for telomerase activity. This is the first TR identified from the previously unexplored invertebrate clade and provides the first glimpse of TR evolution in the deuterostome lineage. Moreover, our TR discovery approach is a significant step toward the comprehensive understanding of telomerase RNP evolution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.