ArticleProceedings of the National Academy of Sciences of the United States of America2013
Exogenous gypsy insulator sequences modulate transgene expression in the malaria vector mosquito, Anopheles stephensi.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Configuring the Code: Enhancer-Promoter Arrangement and Transcriptional Regulation.Journal of molecular biology · 2026Review
- Establishment of CRISPR/Cas9-based knock-in in a hemimetabolous insect: targeted gene tagging in the cricket Gryllus bimaculatus.Development (Cambridge, England) · 2025Article
- Dual effector population modification gene-drive strains of the African malaria mosquitoes,Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Evaluation of qPCR to Detect Shifts in Population Composition of the Rhizobial SymbiontBiology · 2023Article
- The genome trilogy of Anopheles stephensi, an urban malaria vector, reveals structure of a locus associated with adaptation to environmental heterogeneity.Scientific reports · 2022Article
- The impact of transgenesis on mosquito fitness: A review.Frontiers in insect science · 2022Review
- Characterization of a female germline and early zygote promoter from the transcription factor bZip1 in the dengue mosquito Aedes aegypti.Parasites & vectors · 2020Article
- A drug-inducible sex-separation technique for insects.Nature communications · 2020Article
- Experimental population modification of the malaria vector mosquito, Anopheles stephensi.PLoS genetics · 2019Article
- Isolation and identification of Asaia sp. in Anopheles spp. mosquitoes collected from Iranian malaria settings: steps toward applying paratransgenic tools against malaria.Parasites & vectors · 2018Article
- Highly Efficient Site-Specific Mutagenesis in Malaria Mosquitoes Using CRISPR.G3 (Bethesda, Md.) · 2018Article
- Suppressor of hairy-wing, modifier of mdg4 and centrosomal protein of 190 gene orthologues of the gypsy insulator complex in the malaria mosquito, Anopheles stephensi.Insect molecular biology · 2016Article
- Disruption of dengue virus transmission by mosquitoes.Current opinion in insect science · 2015Article
- Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes.Insects · 2015Review
- Novel Genetic and Molecular Tools for the Investigation and Control of Dengue Virus Transmission by Mosquitoes.Current tropical medicine reports · 2014Article
- Development of a population suppression strain of the human malaria vector mosquito, Anopheles stephensi.Malaria journal · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Malaria parasites are transmitted to humans by mosquitoes of the genus Anopheles, and these insects are the targets of innovative vector control programs. Proposed approaches include the use of genetic strategies based on transgenic mosquitoes to suppress or modify vector populations. Although substantial advances have been made in engineering resistant mosquito strains, limited efforts have been made in refining mosquito transgene expression, in particular attenuating the effects of insertions sites, which can result in variations in phenotypes and impacts on fitness due to the random integration of transposon constructs. A promising strategy to mitigate position effects is the identification of insulator or boundary DNA elements that could be used to isolate transgenes from the effects of their genomic environment. We applied quantitative approaches that show that exogenous insulator-like DNA derived from the Drosophila melanogaster gypsy retrotransposon can increase and stabilize transgene expression in transposon-mediated random insertions and recombinase-catalyzed, site-specific integrations in the malaria vector mosquito, Anopheles stephensi. These sequences can contribute to precise expression of transgenes in mosquitoes engineered for both basic and applied goals.
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