ArticleEuropean journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V2013
Stabilization of a recombinant ricin toxin A subunit vaccine through lyophilization.
Article in European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Light-assisted drying enables vaccine stabilization and supports cold-chain-independent distribution.Scientific reports · 2026Article
- Integrated optical and thermal modeling for the development of a scalable multi-sample light-assisted drying platform for biologic stabilization.Frontiers in bioengineering and biotechnology · 2026Article
- Randomized-field microwave-assisted pharmaceutical lyophilization with closed-loop control.Scientific reports · 2025Article
- Clinical and Preclinical Methods of Heat-Stabilization of Human Vaccines.Molecular pharmaceutics · 2024Review
- Strategies for overcoming protein and peptide instability in biodegradable drug delivery systems.Advanced drug delivery reviews · 2023Review
- Serum antibody profiling identifies vaccine-induced correlates of protection against aerosolized ricin toxin in rhesus macaques.NPJ vaccines · 2022Article
- An Experimental Adult Zebrafish Model forMicrobiology spectrum · 2022Article
- Review
- Durable Immunity to Ricin Toxin Elicited by a Thermostable, Lyophilized Subunit Vaccine.mSphere · 2021Article
- Article
- Development of thermostable vaccine adjuvants.Expert review of vaccines · 2021Review
- Lyophilized yeast powder for adjuvant free thermostable vaccine delivery.Applied microbiology and biotechnology · 2021Article
- Impact of Excipients on Stability of Polymer Microparticles for Autoimmune Therapy.Frontiers in bioengineering and biotechnology · 2020Article
- Single-administration, thermostable human papillomavirus vaccines prepared with atomic layer deposition technology.NPJ vaccines · 2020Article
- Thermal Stabilization of Viral Vaccines in Low-Cost Sugar Films.Scientific reports · 2019Article
- Thermostable Ebola virus vaccine formulations lyophilized in the presence of aluminum hydroxide.European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V · 2019Article
- Article
- The immunogenicity of thin-film freeze-dried, aluminum salt-adjuvanted vaccine when exposed to different temperatures.Human vaccines & immunotherapeutics · 2017Article
- Progress and challenges associated with the development of ricin toxin subunit vaccines.Expert review of vaccines · 2016Review
- Recent advances in the development of vaccines against ricin.Human vaccines & immunotherapeutics · 2016Article
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Authors and funding
10 authors.
Funding
Abstract
Lyophilization was used to prepare dry, glassy solid vaccine formulations of recombinant ricin toxin A-chain containing suspensions of colloidal aluminum hydroxide adjuvant. Four lyophilized formulations were prepared by using combinations of rapid or slow cooling during lyophilization and one of two buffers, histidine or ammonium acetate. Trehalose was used as the stabilizing excipient. Aggregation of the colloidal aluminum hydroxide suspension was reduced in formulations processed with a rapid cooling rate. Aluminum hydroxide particle size distributions, glass transition temperatures, water contents, and immunogenicities of lyophilized vaccines were independent of incubation time at 40 °C for up to 15 weeks. Mice immunized with reconstituted ricin toxin subunit A (RTA) vaccines produced RTA-specific antibodies and toxin-neutralizing antibodies (TNAs) regardless of the length of high temperature vaccine storage or the degree of aluminum adjuvant aggregation that occurred during lyophilization. In murine studies, lyophilized formulations of vaccines conferred protection against exposure to lethal doses of ricin, even after the lyophilized formulations had been stored at 40 °C for 4 weeks. A corresponding liquid formulation of vaccine stored at 40 °C elicited RTA-specific antibody titers but failed to confer immunity during a ricin challenge.
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Registered trials
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