ArticleJournal of virological methods2013
Comparison of four multiplex PCR assays for the detection of viral pathogens in respiratory specimens.
Article in Journal of virological methods, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Practical Guidance for Clinical Microbiology Laboratories: Viruses Causing Acute Respiratory Tract Infections.Clinical microbiology reviews · 2019Guideline
- Randomized controlled trial of the effect of regular paracetamol on influenza infection.Respirology (Carlton, Vic.) · 2016Trial
- Performance of novel digital real-time PCR for detection of SARS-CoV-2, respiratory syncytial viruses, and influenza viruses in Ghana.Microbiology spectrum · 2025Article
- A fully integrated duplex RT-LAMP device for the detection of viral infections.Biomedical microdevices · 2023Article
- Comparison of performance between Fast Track Diagnostics Respiratory Kit and the CDC global reference laboratory for influenza rRT-PCR panel for detection of influenza A and influenza B.Influenza and other respiratory viruses · 2021Article
- SARS-CoV, MERS-CoV and SARS-CoV-2: A Diagnostic Challenge.Measurement : journal of the International Measurement Confederation · 2021Article
- [Human coronaviruses].Revue francophone des laboratoires : RFL · 2020Article
- A deep dive into testing and management of COVID-19 for Australian high performance and professional sport.Journal of science and medicine in sport · 2020Article
- Clinical evaluation of AusDiagnostics SARS-CoV-2 multiplex tandem PCR assay.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
- Clinical Evaluation of the cobas SARS-CoV-2 Test and a Diagnostic Platform Switch during 48 Hours in the Midst of the COVID-19 Pandemic.Journal of clinical microbiology · 2020Article
- Cost Impact Model of a Novel Multi-mRNA Host Response Assay for Diagnosis and Risk Assessment of Acute Respiratory Tract Infections and Sepsis in the Emergency Department.Journal of health economics and outcomes research · 2020Article
- Duplex real-time RT-PCR assay for detection and subgroup-specific identification of human respiratory syncytial virus.Journal of virological methods · 2019Article
- Comparison of FTD® respiratory pathogens 33 and a singleplex CDC assay for the detection of respiratory viruses: A study from Cameroon.Diagnostic microbiology and infectious disease · 2019Article
- Effect of stochasticity on coinfection dynamics of respiratory viruses.BMC bioinformatics · 2019Article
- Lower Respiratory Multiplex Panels for the Detection of Bacterial and Viral Infections.Clinical microbiology newsletter · 2018Article
- Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real-Q RV.Journal of clinical laboratory analysis · 2018Article
- Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to SeeplexJournal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy · 2017Article
- Role of multiplex PCR analysis in children with febrile seizures.Wiener medizinische Wochenschrift (1946) · 2017Article
- Missed opportunities for antimicrobial stewardship in pre-school children admitted to hospital with lower respiratory tract infection.Journal of paediatrics and child health · 2017Article
- Recent sequence variation in probe binding site affected detection of respiratory syncytial virus group B by real-time RT-PCR.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiplex PCR has become the test of choice for the detection of multiple respiratory viruses in clinical specimens. However, there are few direct comparisons of different PCR assays. This study compares 4 different multiplex PCR assays for the recovery of common respiratory viruses. We tested 213 respiratory specimens using four different multiplex PCR assays: the xTAG respiratory viral panel fast (Abbott Molecular Laboratories), Fast-track Respiratory Pathogen assay (Fast-track Diagnostics), Easyplex respiratory pathogen 12 kit (Ausdiagnostics), and an in-house multiplex real-time PCR assay. The performance of the four assays was very similar, with 93-100% agreement for all comparisons. Other issues, such as through-put, technical requirements and cost, are likely to be as important for making a decision about which of these assays to use given their comparative performance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.