ArticleNucleic acids research2013
Quantitative in vivo analysis of chromatin binding of Polycomb and Trithorax group proteins reveals retention of ASH1 on mitotic chromatin.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Article
- A maternal Polycomb imprint is maintained in early Drosophila development through lower-order methylation states.Cell reports · 2026Article
- Lower-order methylation states underlie the maintenance and re-establishment of Polycomb modifications inbioRxiv : the preprint server for biology · 2025Article
- A de novo missense variant in EZH1 associated with developmental delay exhibits functional deficits in Drosophila melanogaster.Genetics · 2023Article
- AmedRxiv : the preprint server for health sciences · 2023Article
- Nuclear architecture and the structural basis of mitotic memory.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Review
- The control of transcriptional memory by stable mitotic bookmarking.Nature communications · 2022Article
- The Trithorax group protein ASH1 requires a combination of BAH domain and AT hooks, but not the SET domain, for mitotic chromatin binding and survival.Chromosoma · 2021Article
- Live-cell single particle tracking of PRC1 reveals a highly dynamic system with low target site occupancy.Nature communications · 2021Article
- How do DNA-bound proteins leave their binding sites? The role of facilitated dissociation.Current opinion in chemical biology · 2019Review
- Genetic Dissection Reveals the Role of Ash1 Domains in Counteracting Polycomb Repression.G3 (Bethesda, Md.) · 2019Article
- Theoretical analysis of Polycomb-Trithorax systems predicts that poised chromatin is bistable and not bivalent.Nature communications · 2019Article
- Ash1 counteracts Polycomb repression independent of histone H3 lysine 36 methylation.EMBO reports · 2019Article
- Identification of GA-Binding Protein Transcription Factor Alpha Subunit (GABPA) as a Novel Bookmarking Factor.International journal of molecular sciences · 2019Article
- Temporal control of gene expression by the pioneer factor Zelda through transient interactions in hubs.Nature communications · 2018Article
- Article
- Remembering the past: Mitotic bookmarking in a developing embryo.Current opinion in systems biology · 2018Review
- Coactivators and general transcription factors have two distinct dynamic populations dependent on transcription.The EMBO journal · 2017Article
- Mapping the Dynamics of the Glucocorticoid Receptor within the Nuclear Landscape.Scientific reports · 2017Article
- Causal role for inheritance of H3K27me3 in maintaining the OFF state of aScience (New York, N.Y.) · 2017Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Polycomb (PcG) and Trithorax (TrxG) group proteins work antagonistically on several hundred developmentally important target genes, giving stable mitotic memory, but also allowing flexibility of gene expression states. How this is achieved in quantitative terms is poorly understood. Here, we present a quantitative kinetic analysis in living Drosophila of the PcG proteins Enhancer of Zeste, (E(Z)), Pleiohomeotic (PHO) and Polycomb (PC) and the TrxG protein absent, small or homeotic discs 1 (ASH1). Fluorescence recovery after photobleaching and fluorescence correlation spectroscopy reveal highly dynamic chromatin binding behaviour for all proteins, with exchange occurring within seconds. We show that although the PcG proteins substantially dissociate from mitotic chromatin, ASH1 remains robustly associated with chromatin throughout mitosis. Finally, we show that chromatin binding by ASH1 and PC switches from an antagonistic relationship in interphase, to a cooperative one during mitosis. These results provide quantitative insights into PcG and TrxG chromatin-binding dynamics and have implications for our understanding of the molecular nature of epigenetic memory.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.