ArticleInvestigative ophthalmology & visual science2013
Coherent anti-stokes Raman scattering (CARS) microscopy: a novel technique for imaging the retina.
Article in Investigative ophthalmology & visual science, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Harnessing the power of Raman spectroscopic imaging for ophthalmology.Frontiers in chemistry · 2023Review
- Rapid and label-free detection of COVID-19 using coherent anti-Stokes Raman scattering microscopy.MRS communications · 2020Article
- Volume holographic imaging endoscopic design and construction techniques.Journal of biomedical optics · 2017Article
- Epi-direction detected multimodal imaging of an unstained mouse retina with a Yb-fiber laser.Proceedings of SPIE--the International Society for Optical Engineering · 2017Article
- Microstructure visualization of conventional outflow pathway and finite element modeling analysis of trabecular meshwork.Biomedical engineering online · 2016Article
- Clinical instrumentation and applications of Raman spectroscopy.Chemical Society reviews · 2016Review
- Direct label-free measurement of the distribution of small molecular weight compound inside thick biological tissue using coherent Raman microspectroscopy.Scientific reports · 2015Article
- Coherent Raman Scattering Microscopy in Biology and Medicine.Annual review of biomedical engineering · 2015Review
- Third harmonic generation microscopy of a mouse retina.Molecular vision · 2015Article
- Imaging the effects of prostaglandin analogues on cultured trabecular meshwork cells by coherent anti-stokes Raman scattering.Investigative ophthalmology & visual science · 2013Article
- Imaging the intact mouse cornea using coherent anti-stokes Raman scattering (CARS).Investigative ophthalmology & visual science · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo image the cellular and noncellular structures of the retina in an intact mouse eye without the application of exogenous fluorescent labels using noninvasive, nondestructive techniques.
methodsFreshly enucleated mouse eyes were imaged using two nonlinear optical techniques: coherent anti-Stokes Raman scattering (CARS) and two-photon autofluorescence (TPAF). Cross sectional transverse sections and sequential flat (en face) sagittal sections were collected from a region of sclera approximately midway between the limbus and optic nerve. Imaging proceeded from the surface of the sclera to a depth of ∼60 μm.
resultsThe fluorescent signal from collagen fibers within the sclera was evident in the TPAF channel; the scleral collagen fibers showed no organization and appeared randomly packed. The sclera contained regions lacking TPAF and CARS fluorescence of ∼3 to 15 μm in diameter that could represent small vessels or scleral fibroblasts. Intense punctate CARS signals from the retinal pigment epithelial layer were of a size and shape of retinyl storage esters. Rod outer segments could be identified by the CARS signal from their lipid-rich plasma membranes.
conclusionsCARS microscopy can be used to image the outer regions of the mammalian retina without the use of a fluorescent dye or exogenously expressed recombinant protein. With technical advancements, CARS/TPAF may represent a new avenue for noninvasively imaging the retina and might complement modalities currently used in clinical practice.
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