ArticleDisease models & mechanisms2013
An entirely automated method to score DSS-induced colitis in mice by digital image analysis of pathology slides.
Article in Disease models & mechanisms, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Quality of methods reporting in animal models of colitis.Inflammatory bowel diseases · 2015Pooled it
- A Pilot Study for Establishing DSS-Induced Colitis Model in Minipigs.Tissue engineering and regenerative medicine · 2026Article
- A Proposed Standardized Scoring Equation for Murine Models of Colitis.Digestive diseases and sciences · 2026Article
- Article
- Perinatal tissue-derived exosomes ameliorate colitis in mice by regulating the Foxp3 + Treg cells and gut microbiota.Stem cell research & therapy · 2023Article
- Quantitative Image Analysis for Tissue Biomarker Use: A White Paper From the Digital Pathology Association.Applied immunohistochemistry & molecular morphology : AIMM · 2021Article
- Transpathology: molecular imaging-based pathology.European journal of nuclear medicine and molecular imaging · 2021Review
- Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model.Journal of visualized experiments : JoVE · 2021Article
- Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.Gut microbes · 2020Article
- Effects of Linoleic Acid-Rich Diet on Plasma Profiles of Eicosanoids and Development of Colitis inJournal of agricultural and food chemistry · 2020Article
- Prescription Opioids induce Gut Dysbiosis and Exacerbate Colitis in a Murine Model of Inflammatory Bowel Disease.Journal of Crohn's & colitis · 2020Article
- Recent updates on the basic mechanisms and pathogenesis of inflammatory bowel diseases in experimental animal models.Intestinal research · 2020Review
- Article
- Deciphering the immune microenvironment of a tissue by digital imaging and cognition network.Scientific reports · 2018Article
- Regulatory T-cell depletion in the gut caused by integrin β7 deficiency exacerbates DSS colitis by evoking aberrant innate immunity.Mucosal immunology · 2016Article
- Claudin-3 and occludin tissue content in the glands of colonic mucosa with and without a fecal stream.Journal of molecular histology · 2015Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DSS (dextran sulfate sodium) model of colitis is a mouse model of inflammatory bowel disease. Microscopic symptoms include loss of crypt cells from the gut lining and infiltration of inflammatory cells into the colon. An experienced pathologist requires several hours per study to score histological changes in selected regions of the mouse gut. In order to increase the efficiency of scoring, Definiens Developer software was used to devise an entirely automated method to quantify histological changes in the whole H&E slide. When the algorithm was applied to slides from historical drug-discovery studies, automated scores classified 88% of drug candidates in the same way as pathologists' scores. In addition, another automated image analysis method was developed to quantify colon-infiltrating macrophages, neutrophils, B cells and T cells in immunohistochemical stains of serial sections of the H&E slides. The timing of neutrophil and macrophage infiltration had the highest correlation to pathological changes, whereas T and B cell infiltration occurred later. Thus, automated image analysis enables quantitative comparisons between tissue morphology changes and cell-infiltration dynamics.
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