ArticleMolecular biology of the cell2013
Take advantage of time in your experiments: a guide to simple, informative kinetics assays.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed.
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- A guide RNA repeat checkpoint steers CRISPR-Cas9 catalysis.bioRxiv : the preprint server for biology · 2025Article
- Nonmuscle myosin 2 turnover in cells is synergistically controlled by the tail and the motor domain.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cargo adaptor identity controls the mechanism and kinetics of dynein activation.bioRxiv : the preprint server for biology · 2024Article
- Single-molecule tracking reveals dual front door/back door inhibition of Cel7A cellulase by its product cellobiose.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Light-Activatable MBD-Readers of 5-Methylcytosine Reveal Domain-Dependent Chromatin Association Kinetics In Vivo.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Toxoplasma gondii actin filaments are tuned for rapid disassembly and turnover.Nature communications · 2024Article
- Insulin C-peptide secretion on-a-chip to measure the dynamics of secretion and metabolism from individual islets.Cell reports methods · 2023Article
- Integration Host Factor Binds DNA Holliday Junctions.International journal of molecular sciences · 2022Article
- Multivalent designed proteins neutralize SARS-CoV-2 variants of concern and confer protection against infection in mice.Science translational medicine · 2022Article
- Celebrating 20 years of live single-actin-filament studies with five golden rules.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Measuring microtubule binding kinetics of membrane-bound kinesin motors using supported lipid bilayers.STAR protocols · 2021Article
- Multivalent designed proteins protect against SARS-CoV-2 variants of concern.bioRxiv : the preprint server for biology · 2021Article
- Integrating single-molecule FRET and biomolecular simulations to study diverse interactions between nucleic acids and proteins.Essays in biochemistry · 2021Review
- How to measure and evaluate binding affinities.eLife · 2020Article
- Single gold-bridged nanoprobes for identification of single point DNA mutations.Nature communications · 2019Article
- "Essentially, all models are wrong, but some are useful"-a cross-disciplinary agenda for building useful models in cell biology and biophysics.Biophysical reviews · 2018Review
- Nup159 Weakens Gle1 Binding to Dbp5 But Does Not Accelerate ADP Release.Journal of molecular biology · 2018Article
Corrections and comments
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2 authors.
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Abstract
Understanding virtually any process in cellular and molecular biology depends on knowledge of the rates of the biochemical reactions, so it is regrettable that few cellular and molecular biologists take advantage of kinetics experiments in their work. Fortunately, the kinetics experiments that are most useful for understanding cellular systems are within reach for everyone whose research would benefit from this information. This essay describes simple methods to measure the valuable kinetic parameters that characterize the dynamics of life processes. These "transient-state" methods not only differ in concept from traditional approaches used to analyze enzyme reactions at steady state, but they are also applicable to learning about the dynamics of any biological process.
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