ArticleJournal of fluorescence2013
Comparison of in vivo optical systems for bioluminescence and fluorescence imaging.
Article in Journal of fluorescence, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Fluorescent Labeling Strategies for Tracking Micro- and Nanoplastics in Biological Systems.Materials (Basel, Switzerland) · 2026Review
- Controlled Release of the IL-1R Antagonist, Anakinra, from Microgels with an Extended Intra-articular Residence Time.Pharmaceutical research · 2026Article
- Tracking adipose-derived mesenchymal stromal cells in the eye: Integrating IVIS imaging and Alu PCR for enhanced detection of human cells.Regenerative therapy · 2025Article
- Flow cytometric analysis of the murine placenta to evaluate nanoparticle platforms during pregnancy.Placenta · 2025Article
- BrightMice: a low-cost do-it-yourself instrument, designed for in vivo fluorescence mouse imaging.Scientific reports · 2024Article
- Two-color diffuseJournal of biomedical optics · 2024Article
- Orthotopic and metastatic tumour models in preclinical cancer research.Pharmacology & therapeutics · 2024Review
- Plasmonic silver and gold nanoparticles: shape- and structure-modulated plasmonic functionality for point-of-caring sensing, bio-imaging and medical therapy.Chemical Society reviews · 2024Review
- Regulatory guidelines and preclinical tools to study the biodistribution of RNA therapeutics.Advanced drug delivery reviews · 2022Review
- Visible-light and near-infrared fluorescence and surface-enhanced Raman scattering point-of-care sensing and bio-imaging: a review.Chemical Society reviews · 2022Review
- Red-shifted click beetle luciferase mutant expands the multicolor bioluminescent palette for deep tissue imaging.iScience · 2021Article
- Evading the host response: Staphylococcus "hiding" in cortical bone canalicular system causes increased bacterial burden.Bone research · 2020Article
- Cholic Acid-Peptide Conjugates as Potent Antimicrobials against Interkingdom Polymicrobial Biofilms.Antimicrobial agents and chemotherapy · 2019Article
- Near-infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin.eLife · 2019Article
- QuantitativeNeurophotonics · 2019Article
- New insights into experimental visceral leishmaniasis: Real-time in vivo imaging of Leishmania donovani virulence.PLoS neglected tropical diseases · 2017Article
- Use of luciferase probes to measure ATP in living cells and animals.Nature protocols · 2017Article
- Ultra-sensitive in-situ detection of near-infrared persistent luminescent tracer nanoagents in crude oil-water mixtures.Scientific reports · 2016Article
- Radiation-Guided Peptide Delivery in a Mouse Model of Nasopharyngeal Carcinoma.BioMed research international · 2016Article
- Targeting tumor hypoxia: a third generation 2-nitroimidazole-indocyanine dye-conjugate with improved fluorescent yield.Organic & biomolecular chemistry · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo optical imaging has become a popular tool in animal laboratories. Currently, many in vivo optical imaging systems are available on the market, which often makes it difficult for research groups to decide which system fits their needs best. In this work we compared different commercially available systems, which can measure both bioluminescent and fluorescent light. The systems were tested for their bioluminescent and fluorescent sensitivity both in vitro and in vivo. The IVIS Lumina II was found to be most sensitive for bioluminescence imaging, with the Photon Imager a close second. Contrary, the Kodak system was, in vitro, the most sensitive system for fluorescence imaging. In vivo, the fluorescence sensitivity of the systems was similar. Finally, we examined the added value of spectral unmixing algorithms for in vivo optical imaging and demonstrated that spectral unmixing resulted in at least a doubling of the in vivo sensitivity. Additionally, spectral unmixing also enabled separate imaging of dyes with overlapping spectra which were, without spectral unmixing, not distinguishable.
Indexed as
Identifiers
23579930What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.