ArticleMemorias do Instituto Oswaldo Cruz2013
Comparative analyses of classical phenotypic method and ribosomal RNA gene sequencing for identification of medically relevant Candida species.
Article in Memorias do Instituto Oswaldo Cruz, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Phenotypic and Molecular Assessment ofPathogens (Basel, Switzerland) · 2026Article
- Article
- Translation elongation factor 1-alpha gene as a marker for diagnosing of candidal onychomycosis.Current medical mycology · 2020Article
- Prevalence of human pathogens of the clade Nakaseomyces in a culture collection-the first report on Candida bracarensis in Poland.Folia microbiologica · 2019Article
- Identification and Antifungal Susceptibility Profiles ofFrontiers in microbiology · 2017Article
- Candida glabrata species complex prevalence and antifungal susceptibility testing in a culture collection: First description of Candida nivariensis in Argentina.Mycopathologia · 2016Article
- Identification and Antifungal Susceptibility Profiles of Candida haemulonii Species Complex Clinical Isolates from a Multicenter Study in China.Journal of clinical microbiology · 2016Article
- First Case of Fungemia Due to Pseudozyma aphidis in a Pediatric Patient with Osteosarcoma in Latin America.Journal of clinical microbiology · 2015Article
- Three clustered cases of candidemia caused by Candida quercitrusa and mycological characteristics of this novel species.Journal of clinical microbiology · 2014Article
- Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland.Memorias do Instituto Oswaldo Cruz · 2014Article
- Phylogenetic relationships matter: antifungal susceptibility among clinically relevant yeasts.Antimicrobial agents and chemotherapy · 2014Article
- Article
- Susceptibility and molecular characterization of Candida species from patients with vulvovaginitis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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7 authors.
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Abstract
As the distribution of Candida species and their susceptibility to antifungal agents have changed, a new means of accurately and rapidly identifying these species is necessary for the successful early resolution of infection and the subsequent reduction of morbidity and mortality. The current work aimed to evaluate ribosomal RNA gene sequencing for the identification of medically relevant Candida species in comparison with a standard phenotypic method. Eighteen reference strains (RSs), 69 phenotypically identified isolates and 20 inconclusively identified isolates were examined. Internal transcribed spaces (ITSs) and D1/D2 of the 26S ribosomal RNA gene regions were used as targets for sequencing. Additionally, the sequences of the ITS regions were used to establish evolutionary relationships. The sequencing of the ITS regions was successful for 88% (94/107) of the RS and isolates, whereas 100% of the remaining 12% (13/107) of the samples were successfully analysed by sequencing the D1/D2 region. Similarly, genotypic analysis identified all of the RS and isolates, including the 20 isolates that were not phenotypically identified. Phenotypic analysis, however, misidentified 10% (7/69) of the isolates. Phylogenetic analysis allowed the confirmation of the relationships between evolutionarily close species. Currently, the use of genotypic methods is necessary for the correct identification of Candida species.
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