ArticleNature protocols2013
Microinjection of mRNA or morpholinos for reverse genetic analysis in the starlet sea anemone, Nematostella vectensis.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed.
- c-JUN controls microbial colonization via selective phagocytosis in the sea anemone Nematostella.Nature communications · 2026Article
- Lineage-specific elaboration of a conserved cnidogenic program drives cnidocyte diversification and illuminates cell type evolution.Research square · 2026Article
- Hypoxia-induced gene expression changes in N. vectensis embryos.Scientific reports · 2026Article
- NvashA function reveals temporal differences in neural subtype generation in cnidarians.Scientific reports · 2026Article
- Characterization of RNA interference in the cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification.PLoS biology · 2026Article
- Whole body regeneration deploys a rewired embryonic gene regulatory network logic.Nature communications · 2025Article
- Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata.EvoDevo · 2025Article
- Capitella teleta gets left out: possible evolutionary shift causes loss of left tissues rather than increased neural tissue from dominant-negative BMPR1.Neural development · 2024Article
- A noveliScience · 2024Article
- Cytoplasmic Polyadenylation Is an Ancestral Hallmark of Early Development in Animals.Molecular biology and evolution · 2023Article
- The brain regulatory program predates central nervous system evolution.Scientific reports · 2023Article
- Cell Membrane-Camouflaged Nanoparticles Mediated Nucleic Acids Delivery.International journal of nanomedicine · 2023Review
- Review
- Creating a User-Friendly and Open-Access Gene Expression Database for Comparing Embryonic Development and Regeneration in Nematostella vectensis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The cyclic dinucleotide 2'3'-cGAMP induces a broad antibacterial and antiviral response in the sea anemoneProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Review
- Transcriptomic Analysis in the Sea Anemone Nematostella vectensis.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Cytoskeletal and synaptic polarity of LWamide-like+ ganglion neurons in the sea anemoneThe Journal of experimental biology · 2020Article
- TRIM28 regulates sprouting angiogenesis through VEGFR-DLL4-Notch signaling circuit.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We describe a protocol for microinjection of embryos for an emerging model system, the cnidarian sea anemone, Nematostella vectensis. In addition, we provide protocols for carrying out overexpression and knockdown of gene function through microinjection of in vitro-translated mRNAs or gene-specific oligonucleotide morpholinos (MOs), respectively. Our approach is simple, and it takes advantage of the natural adherence properties of the early embryo to position them in a single layer on a polystyrene dish. Embryos are visualized on a dissecting microscope equipped with epifluorescence and injected with microinjection needles using a picospritzer forced-air injection system. A micromanipulator is used to guide the needle to impale individual embryos. Injection takes ∼1.5 h, and an experienced researcher can inject ∼2,000 embryos in a single session. With the availability of the published Nematostella genome, the entire protocol, including cloning and transcription of mRNAs, can be carried out in ∼1 week.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.