ArticleNature protocols2013
Characterizing the spatiotemporal expression of RNAs and proteins in the starlet sea anemone, Nematostella vectensis.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 88 citations in OpenAlex.
- Extreme conservation of cnidarian stinging cell identity despite 600 million years of evolution.bioRxiv : the preprint server for biology · 2026Article
- Lineage-specific elaboration of a conserved cnidogenic program drives cnidocyte diversification and illuminates cell type evolution.Research square · 2026Article
- Hypoxia-induced gene expression changes in N. vectensis embryos.Scientific reports · 2026Article
- NvashA function reveals temporal differences in neural subtype generation in cnidarians.Scientific reports · 2026Article
- Article
- Sleeping upside-down: Knockdown of a sleep-associated gene induces daytime sleep in the jellyfishProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Microinjection, gene knockdown, and CRISPR-mediated gene knock-in in the hard coral, Astrangia poculata.EvoDevo · 2025Article
- LRRK2 kinase activity is necessary for development and regeneration in Nematostella vectensis.Neural development · 2024Article
- Gene Regulatory Network that Shaped the Evolution of Larval Apical Organ in Cnidaria.Molecular biology and evolution · 2024Article
- Fluorescence In Situ Hybridization as a Tool for Studying the Specification and Differentiation of Cell Types in Nematostella vectensis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- SABER-FISH in Hydractinia.Methods in molecular biology (Clifton, N.J.) · 2024Article
- LRRK2 kinase activity is necessary for development and regeneration in Nematostella vectensis.Research square · 2023Article
- Nematostella vectensis exemplifies the exceptional expansion and diversity of opsins in the eyeless Hexacorallia.EvoDevo · 2023Article
- The brain regulatory program predates central nervous system evolution.Scientific reports · 2023Article
- Single-cell atavism reveals an ancient mechanism of cell type diversification in a sea anemone.Nature communications · 2023Article
- Gene Loss may have Shaped the Cnidarian and Bilaterian Hox and ParaHox Complement.Genome biology and evolution · 2023Article
- Molecular and cellular architecture of the larval sensory organ in the cnidarian Nematostella vectensis.Development (Cambridge, England) · 2022Article
- Article
- Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The cyclic dinucleotide 2'3'-cGAMP induces a broad antibacterial and antiviral response in the sea anemoneProceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
In an effort to reconstruct the early evolution of animal genes and proteins, there is an increasing focus on basal animal lineages such as sponges, cnidarians, ctenophores and placozoans. Among the basal animals, the starlet sea anemone Nematostella vectensis (phylum Cnidaria) has emerged as a leading laboratory model organism partly because it is well suited to experimental techniques for monitoring and manipulating gene expression. Here we describe protocols adapted for use in Nematostella to characterize the expression of RNAs by in situ hybridization using either chromogenic or fluorescence immunohistochemistry (∼1 week), as well as to characterize protein expression by whole-mount immunofluorescence (∼3 d). We also provide a protocol for labeling cnidocytes (∼3 h), the phylum-specific sensory-effector cell type that performs a variety of functions in cnidarians, including the delivery of their venomous sting.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.