ArticleNature protocols2013
Isolation of DNA, RNA and protein from the starlet sea anemone Nematostella vectensis.
Article in Nature protocols, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
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- Chromosome-level genome assemblies of 2 hemichordates provide new insights into deuterostome origin and chromosome evolution.PLoS biology · 2024Article
- Molecular tuning of sea anemone stinging.eLife · 2023Article
- Effects ofBiology · 2022Article
- Polymorphism in the symmetries of gastric pouch arrangements in the sea anemone D. lineata.Zoological letters · 2021Article
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- Altered Immune Landscape and Disrupted Coral-Frontiers in physiology · 2019Article
- Possible roles of glutamine synthetase in responding to environmental changes in a scleractinian coral.Molecular biology reports · 2018Article
- Transcriptome, expression, and activity analyses reveal a vital heat shock protein 70 in the stress response of stony coral Pocillopora damicornis.Cell stress & chaperones · 2018Article
- Article
- A transcriptional time-course analysis of oral vs. aboral whole-body regeneration in the Sea anemone Nematostella vectensis.BMC genomics · 2016Article
- Evolution of the perlecan/HSPG2 gene and its activation in regenerating Nematostella vectensis.PloS one · 2015Article
- Production of a reference transcriptome and transcriptomic database (EdwardsiellaBase) for the lined sea anemone, Edwardsiella lineata, a parasitic cnidarian.BMC genomics · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Among marine invertebrates, the starlet sea anemone Nematostella vectensis has emerged as an important laboratory model system. One advantage of working with this species relative to many other marine invertebrates is the ease of isolating relatively pure DNA, RNA and protein. Nematostella can be raised at high densities, under clean culture conditions, and it lacks integumentary or skeletal structures that can impede the recovery of DNA, RNA or protein. Here we describe methods used in our lab to isolate DNA, RNA and protein from Nematostella embryos, larvae and adults. The methods described here are less expensive than commercial kits and are more easily scalable to larger tissue amounts. Preparation of DNA can be completed in ∼7 h, RNA preparation in ∼1.5 h and protein preparation in ∼1 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.