ArticleBMC veterinary research2013
Comparison of the MGIT 960, BACTEC 460 TB and solid media for isolation of Mycobacterium bovis in United States veterinary specimens.
Article in BMC veterinary research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Accuracy of Tests for Diagnosis of Animal Tuberculosis: Moving Away from the Golden Calf (and towards Bayesian Models).Transboundary and emerging diseases · 2023Pooled it
- Zoonotic tuberculosis: When diagnostic and surveillance systems are not designed to detect it.One health (Amsterdam, Netherlands) · 2026Article
- Risk Factors Influencing the Specificity of Skin Tests for Bovine Tuberculosis in a Low-Prevalence Region.International journal of veterinary science and medicine · 2026Article
- Detection ofFrontiers in microbiology · 2024Article
- Vaccination of White-Tailed Deer withMicroorganisms · 2023Article
- Whole Genome Sequencing LinksFrontiers in veterinary science · 2021Article
- Review of Diagnostic Tests for Detection ofFrontiers in veterinary science · 2021Review
- Effects of InactivatedPathogens (Basel, Switzerland) · 2020Article
- A rare case of spinal tuberculosis due toIDCases · 2020Article
- Characteristics of subclinicalJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2019Article
- Validation of a Real-Time PCR for the Detection ofFrontiers in veterinary science · 2019Article
- Whole Genome Sequencing ofFrontiers in veterinary science · 2018Article
- Diagnosis of active tuberculosis disease: From microscopy to molecular techniques.Journal of clinical tuberculosis and other mycobacterial diseases · 2016Review
- Article
- Article
- Clinical and diagnostic developments of a gamma interferon release assay for use in bovine tuberculosis control programs.Clinical and vaccine immunology : CVI · 2013Article
- Isolation of mycobacteria from clinical samples collected in the United States from 2004 to 2011.BMC veterinary research · 2013Article
- Comparative study of Mycobacterium bovis primary isolation methods.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacteriologic culture remains one of the most important methods to diagnose bovine tuberculosis despite the lengthy incubation time, significant decontamination and media expense, and high biocontainment requirements. Media selection is an important determination of culture sensitivity, and the planned discontinuation of the BACTEC 460 TB culture system has challenged veterinary diagnostic laboratories to evaluate alternatives. At the National Veterinary Services Laboratories the BACTEC MGIT 960 and 4 solid media formulations were compared with the BACTEC 460 TB system on 6,795 veterinary diagnostic specimens submitted for Mycobacterium bovis culture.
resultsM. bovis was isolated from 2.6% of the samples and atypical mycobacteria from 4.4% of the samples. The BACTEC 12B media isolated significantly more M. bovis (93.1% of positive samples) than MGIT 960 media (81.9%). However, contamination rates were much higher for the MGIT media, 17-24%, compared to 7% for BACTEC, suggesting that contamination was a major cause of MGIT reduced sensitivity. Time to signal positive was 2.37 weeks (95% CI 2.24-2.5) for the MGIT, and 3.2 weeks (95% CI 3.07-3.3) for the BACTEC, both earlier than any solid media. Mycobactosel LJ failed to isolate M. bovis from primary culture. An in-house 7H11 media supplemented with calf sera, hemolyzed blood, malachite green and pyruvate recovered more M. bovis (80.6%) with the least amount of contamination of any other solid media evaluated.
conclusionDecontamination methods may have to be optimized and or MGIT media may have to be altered to reduce contamination in veterinary samples. Despite these issues, the MGIT 960 system is still favored over the use of solid media due to decreased time to recovery and the potential for higher sensitivity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.