Evidence map›Paper›PMID 23577305›Full record

ArticleCell journal2013

Cloning, Expression, and in vitro Functional Activity Assay of phiC31 Integrase cDNA in Escherichia coli.

Mohammad Hadi Sekhavati, Mojtaba Tahmoorespur, Kamran Ghaedi, Kianoush Dormiani, Mohammad Reza Nassiri, Yahya Khazaie, Mahboubeh Foruzanfar, Morteza Hosseini, Mohammad Hossein Nasr Esfahani

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Article in Cell journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Mohammad Hadi Sekhavati1. Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Mojtaba Tahmoorespur
Kamran Ghaedi
Kianoush Dormiani
Mohammad Reza Nassiri
Yahya Khazaie
Mahboubeh Foruzanfar
Morteza Hosseini
Mohammad Hossein Nasr Esfahani

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe aim of present study was cloning and expression of phiC31 integrase cDNA in a bacterial expression vector. Thus, an intra molecular assay vector was applied to show in vitro activity of recombinant protein. MATERIALS AND

methodsIn this experimental study, phiC31 cDNA was subcloned into a prokaryotic expression vector and transformed into E.coli Bl21 (DE3). Recombinant phiC31 integrase was purified form the bacterial cell lysates and its activity was verified by an in vitro functional assessment.

resultsSodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the purified phiC31 integrase confirmed the size of protein (70 kDa). Finally, the functionality of purified phiC31 integrase was verified.

conclusionThe results of this study indicated that the purified integrase has a great potential application for in vitro site-specific integration.

Indexed as

E.coli BL21 (DE3)phiC31Site-Specific Integration

Identifiers

PMID23577305
PMCPMC3593930

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.