ArticlePloS one2013
Development of a plastic embedding method for large-volume and fluorescent-protein-expressing tissues.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
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- Structural brain preservation: a potential bridge to future medical technologies.Frontiers in medical technology · 2024Article
- Whole-brain Optical Imaging: A Powerful Tool for Precise Brain Mapping at the Mesoscopic Level.Neuroscience bulletin · 2023Review
- Cryo-fluorescence micro-optical sectioning tomography for volumetric imaging of various whole organs with subcellular resolution.iScience · 2022Article
- Reward ameliorates depressive-like behaviors via inhibition of the substantia innominata to the lateral habenula projection.Science advances · 2022Article
- Multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain.Frontiers in neuroscience · 2022Article
- Three-dimensional visualization of heart-wide myocardial architecture and vascular network simultaneously at single-cell resolution.Frontiers in cardiovascular medicine · 2022Article
- Precision vibratome for high-speed ultrathin biotissue cutting and organ-wide imaging.iScience · 2021Article
- Single-cell morphological characterization of CRH neurons throughout the whole mouse brain.BMC biology · 2021Article
- High-definition imaging using line-illumination modulation microscopy.Nature methods · 2021Article
- Continuous imaging of large-volume tissues with a machinable optical clearing method at subcellular resolution.Biomedical optics express · 2020Article
- Scalable Resin Embedding Method for Large-Volume Brain Tissues with High Fluorescence Preservation Capacity.iScience · 2020Article
- Anatomically revealed morphological patterns of pyramidal neurons in layer 5 of the motor cortex.Scientific reports · 2020Article
- Beyond the whole-mount phenotype: high-resolution imaging in fluorescence-based applications on zebrafish.Biology open · 2019Article
- On-line optical clearing method for whole-brain imaging in mice.Biomedical optics express · 2019Article
- Simultaneous Acquisition of Multicolor Information From Neural Circuits in Resin-Embedded Samples.Frontiers in neuroscience · 2018Article
- High axial resolution imaging system for large volume tissues using combination of inclined selective plane illumination and mechanical sectioning.Biomedical optics express · 2017Article
- A platform for stereological quantitative analysis of the brain-wide distribution of type-specific neurons.Scientific reports · 2017Article
- Plastic embedding immunolabeled large-volume samples for three-dimensional high-resolution imaging.Biomedical optics express · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent proteins serve as important biomarkers for visualizing both subcellular organelles in living cells and structural and functional details in large-volume tissues or organs. However, current techniques for plastic embedding are limited in their ability to preserve fluorescence while remaining suitable for micro-optical sectioning tomography of large-volume samples. In this study, we quantitatively evaluated the fluorescence preservation and penetration time of several commonly used resins in a Thy1-eYFP-H transgenic whole mouse brain, including glycol methacrylate (GMA), LR White, hydroxypropyl methacrylate (HPMA) and Unicryl. We found that HMPA embedding doubled the eYFP fluorescence intensity but required long durations of incubation for whole brain penetration. GMA, Unicryl and LR White each penetrated the brain rapidly but also led to variable quenching of eYFP fluorescence. Among the fast-penetrating resins, GMA preserved fluorescence better than LR White and Unicryl. We found that we could optimize the GMA formulation by reducing the polymerization temperature, removing 4-methoxyphenol and adjusting the pH of the resin solution to be alkaline. By optimizing the GMA formulation, we increased percentage of eYFP fluorescence preservation in GMA-embedded brains nearly two-fold. These results suggest that modified GMA is suitable for embedding large-volume tissues such as whole mouse brain and provide a novel approach for visualizing brain-wide networks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.