ArticleSoft matter2013
Microrheology of highly crosslinked microtubule networks is dominated by force-induced crosslinker unbinding.
Article in Soft matter, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 48 citations in OpenAlex.
- Crosslinker identity and dynamics regulate F-actin network structure and mechanics.Frontiers in cell and developmental biology · 2026Article
- Microrheology: From Video Microscopy to Optical Tweezers.Micromachines · 2025Review
- How cytoskeletal crosstalk makes cells move: Bridging cell-free and cell studies.Biophysics reviews · 2024Review
- Mechanical resilience of the sessile tunicate Botryllus schlosseri.The Journal of experimental biology · 2023Article
- Multiscale architecture: Mechanics of composite cytoskeletal networks.Biophysics reviews · 2022Review
- Passive and Active Microrheology for Biomedical Systems.Frontiers in bioengineering and biotechnology · 2022Review
- Motor-Driven Restructuring of Cytoskeleton Composites Leads to Tunable Time-Varying Elasticity.ACS macro letters · 2021Article
- Cell mechanical properties of human breast carcinoma cells depend on temperature.Scientific reports · 2021Article
- Subtle changes in crosslinking drive diverse anomalous transport characteristics in actin-microtubule networks.Soft matter · 2021Article
- A NanoFE simulation-based surrogate machine learning model to predict mechanical functionality of protein networks from live confocal imaging.Computational and structural biotechnology journal · 2020Article
- Topology-dependent anomalous dynamics of ring and linear DNA are sensitive to cytoskeleton crosslinking.Science advances · 2019Article
- Non-monotonic dependence of stiffness on actin crosslinking in cytoskeleton composites.Soft matter · 2019Article
- Varying crosslinking motifs drive the mesoscale mechanics of actin-microtubule composites.Scientific reports · 2019Article
- Co-Entangled Actin-Microtubule Composites Exhibit Tunable Stiffness and Power-Law Stress Relaxation.Biophysical journal · 2018Article
- Computational modeling of single-cell mechanics and cytoskeletal mechanobiology.Wiley interdisciplinary reviews. Systems biology and medicine · 2018Review
- Mechanical Properties of the Cytoskeleton and Cells.Cold Spring Harbor perspectives in biology · 2017Review
- Nonlinear Actin Deformations Lead to Network Stiffening, Yielding, and Nonuniform Stress Propagation.Biophysical journal · 2017Article
- Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots.Chemical Society reviews · 2017Review
- A multiplexed magnetic tweezer with precision particle tracking and bi-directional force control.Journal of biological engineering · 2017Article
- Foam-like compression behavior of fibrin networks.Biomechanics and modeling in mechanobiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We determine the time- and force-dependent viscoelastic responses of reconstituted networks of microtubules that have been strongly crosslinked by biotin-streptavidin bonds. To measure the microscale viscoelasticity of such networks, we use a magnetic tweezers device to apply localized forces. At short time scales, the networks respond nonlinearly to applied force, with stiffening at small forces, followed by a reduction in the stiffening response at high forces, which we attribute to the force-induced unbinding of crosslinks. At long time scales, force-induced bond unbinding leads to local network rearrangement and significant bead creep. Interestingly, the network retains its elastic modulus even under conditions of significant plastic flow, suggesting that crosslinker breakage is balanced by the formation of new bonds. To better understand this effect, we developed a finite element model of such a stiff filament network with labile crosslinkers obeying force-dependent Bell model unbinding dynamics. The coexistence of dissipation, due to bond breakage, and the elastic recovery of the network is possible because each filament has many crosslinkers. Recovery can occur as long as a sufficient number of the original crosslinkers are preserved under the loading period. When these remaining original crosslinkers are broken, plastic flow results.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.