ArticleTheScientificWorldJournal2013
Sponge-like: a new protocol for preparing bacterial ghosts.
Article in TheScientificWorldJournal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 91 citations in OpenAlex.
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- A Simple and Rapid Method of Probiotic Bacterial Ghost Cell Preparation to Deliver Mycobacterium tuberculosis Antigen.Molecular biotechnology · 2025Article
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- Surface Display of Cholera Toxin B Subunit RecombinantMicroorganisms · 2024Article
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- Bacteria and Bacterial Components as Natural Bio-Nanocarriers for Drug and Gene Delivery Systems in Cancer Therapy.Pharmaceutics · 2023Review
- Preparation of Escherichia coli ghost of anchoring bovine Pasteurella multocida OmpH and its immunoprotective effect.BMC veterinary research · 2023Article
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- Efficient Robust Yield Method for Preparing Bacterial Ghosts byBioengineering (Basel, Switzerland) · 2022Article
- Bacterial Ghosts ofVaccines · 2022Article
- Evaluation of the anticandidal activity of clotrimazole using Lactobacillus caseie ghosts as biological drug carrier.Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences · 2022Article
- Immunological characterization of the chemically prepared ghosts of Salmonella Typhimurium as a vaccine candidate.BMC veterinary research · 2022Article
- Protective Immunity againstInternational journal of molecular sciences · 2022Article
- Hydrochloric acid-treatedMolecular & cellular toxicology · 2022Article
- Bacteria from Infectious Particles to Cell Based Anticancer Targeted Drug Delivery Systems.Pharmaceutics · 2021Review
- Bacterial Ghosts-Based Vaccine and Drug Delivery Systems.Pharmaceutics · 2021Review
- Recent Approaches towards Control of Fungal Diseases in Plants: An Updated Review.Journal of fungi (Basel, Switzerland) · 2021Review
- Article
- The Development of a Phytopathogenic Fungi Control Trial:TheScientificWorldJournal · 2021Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial Ghosts (BGs) received an increasing interest in the recent years for their promising medicinal and pharmaceutical applications. In this study, for the first time we introduce a new protocol for BGs production. E. coli BL21 (DE3) pLysS (Promega) was used as a model to establish a general protocol for BGs preparation. The protocol is based on using active chemical compounds in concentrations less than the Minimum Inhibition Concentration (MIC). Those chemical compounds are SDS, NaOH, and H2O2. Plackett-Burman experimental design was used to map the best conditions for BGs production. Normal and electronic microscopes were used to evaluate the BGs quality (BGQ). Spectrophotometer was used to evaluate the amount of the released protein and DNA. Agarose gel electrophoresis was used to determine the existence of any residue of DNA after each BGs preparation. Viable cells, which existed after running this protocol, were subjected to lysis by inducing the lysozyme gene carried on pLysS plasmid. This protocol is able to produce BGs that can be used in different biotechnological applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.