ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2013
Selective inhibition of vagal afferent nerve pathways regulating cough using Nav 1.7 shRNA silencing in guinea pig nodose ganglia.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 51 citations in OpenAlex.
- Wired for immunity: neuroimmune control of the lung by sensory neurons.Nature reviews. Neuroscience · 2026Review
- Reflex regulation of respiration and heart rate by inhaled activators of vagal bronchopulmonary afferents.Journal of neurophysiology · 2025Article
- Neural Mechanisms Underlying the Coughing Reflex.Neuroscience bulletin · 2023Review
- Neural control of the lower airways: Role in cough and airway inflammatory disease.Handbook of clinical neurology · 2022Review
- Antitussive effects of NaEuropean journal of pharmacology · 2021Article
- Fatigue in Women with Fibromyalgia: A Gene-Physical Activity Interaction Study.Journal of clinical medicine · 2021Article
- Mechanisms and Rationale for Targeted Therapies in Refractory and Unexplained Chronic Cough.Clinical pharmacology and therapeutics · 2021Review
- Molecular identity, anatomy, gene expression and function of neural crest vs. placode-derived nociceptors in the lower airways.Neuroscience letters · 2021Review
- Design and rationale of two phase 3 randomised controlled trials (COUGH-1 and COUGH-2) of gefapixant, a P2X3 receptor antagonist, in refractory or unexplained chronic cough.ERJ open research · 2020Article
- Peripheral and central mechanisms of cough hypersensitivity.Journal of thoracic disease · 2020Review
- Review
- The Prospect for Potent Sodium Voltage-Gated Channel Blockers to Relieve an Excessive Cough.Physiological research · 2020Review
- Molecular/Ionic Basis of Vagal Bronchopulmonary C-Fiber Activation by Inflammatory Mediators.Physiology (Bethesda, Md.) · 2020Review
- Mining the NaBiochemical pharmacology · 2019Review
- Different role of TTX-sensitive voltage-gated sodium channel (NaThe Journal of physiology · 2018Article
- Opposing effects of bronchopulmonary C-fiber subtypes on cough in guinea pigs.American journal of physiology. Regulatory, integrative and comparative physiology · 2018Article
- Control of Neurotransmission by NaV1.7 in Human, Guinea Pig, and Mouse Airway Parasympathetic Nerves.The Journal of pharmacology and experimental therapeutics · 2017Article
- Role of reactive oxygen species and TRP channels in the cough reflex.Cell calcium · 2016Review
- Vagal Afferent Innervation of the Airways in Health and Disease.Physiological reviews · 2016Review
- Pharmacology of Bradykinin-Evoked Coughing in Guinea Pigs.The Journal of pharmacology and experimental therapeutics · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Adeno-associated virus delivery systems and short hairpin RNA (shRNA) were used to selectively silence the voltage-gated sodium channel NaV 1.7 in the nodose ganglia of guinea pigs. The cough reflex in these animals was subsequently assessed. NaV 1.7 shRNA was delivered to the majority of nodose ganglia neurons [50-60% transfection rate determined by green fluorescent protein (GFP) gene cotransfection] and action potential conduction in the nodose vagal nerve fibers, as evaluated using an extracellular recording technique, was markedly and significantly reduced. By contrast, <5% of neurons in the jugular vagal ganglia neurons were transfected, and action potential conduction in the jugular vagal nerve fibers was unchanged. The control virus (with GFP expression) was without effect on action potential discharge and conduction in either ganglia. In vivo, NaV 1.7 silencing in the nodose ganglia nearly abolished cough evoked by mechanically probing the tracheal mucosa in anesthetized guinea pigs. Stimuli such as capsaicin and bradykinin that are known to stimulate both nodose and jugular C-fibers evoked coughing in conscious animals was unaffected by NaV 1.7 silencing in the nodose ganglia. Nodose C-fiber selective stimuli including adenosine, 2-methyl-5-HT, and ATP all failed to evoke coughing upon aerosol challenge. These results indicate that cough is independently regulated by two vagal afferent nerve subtypes in guinea pigs, with nodose Aδ fibers regulating cough evoked mechanically from the trachea and bradykinin- and capsaicin-evoked cough regulated by C-fibers arising from the jugular ganglia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.