ReviewBiopolymers2014
Chemical strategies to understand the language of ubiquitin signaling.
Review in Biopolymers, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Synthesis of ubiquitinated proteins for biochemical and functional analysis.Chemical science · 2023Review
- Combining Chemical Protein Synthesis and Random Nonstandard Peptides Integrated Discovery for Modulating Biological Processes.Accounts of chemical research · 2023Article
- The semisynthesis of site-specifically modified histones and histone-based probes of chromatin-modifying enzymes.Methods (San Diego, Calif.) · 2023Article
- Structure-Uptake Relationship Study of DABCYL Derivatives Linked to Cyclic Cell-Penetrating Peptides for Live-Cell Delivery of Synthetic Proteins.Angewandte Chemie (International ed. in English) · 2022Article
- Posttranslational Chemical Mutagenesis Methods to Insert Posttranslational Modifications into Recombinant Proteins.Molecules (Basel, Switzerland) · 2022Review
- Enhanced Live-Cell Delivery of Synthetic Proteins Assisted by Cell-Penetrating Peptides Fused to DABCYL.Angewandte Chemie (International ed. in English) · 2021Article
- Facile Semisynthesis of Ubiquitylated Peptides with the Ligation Auxiliary 2-Aminooxyethanethiol.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Total chemical synthesis of ester-linked ubiquitinated proteins unravels their behavior with deubiquitinases.Chemical science · 2018Article
- Biological and Physicochemical Functions of Ubiquitylation Revealed by Synthetic Chemistry Approaches.International journal of molecular sciences · 2017Review
- Activity-based probes for the ubiquitin conjugation-deconjugation machinery: new chemistries, new tools, and new insights.The FEBS journal · 2017Review
- A Facile N-Mercaptoethoxyglycinamide (MEGA) Linker Approach to Peptide Thioesterification and Cyclization.Journal of the American Chemical Society · 2017Article
- Selenocysteine as a Latent Bioorthogonal Electrophilic Probe for Deubiquitylating Enzymes.Journal of the American Chemical Society · 2016Article
- Aromatic thiol-mediated cleavage of N-O bonds enables chemical ubiquitylation of folded proteins.Nature communications · 2016Article
- Chemical ubiquitination for decrypting a cellular code.The Biochemical journal · 2016Review
- Synthesis of a Bis-thio-acetone (BTA) Analogue of the Lysine Isopeptide Bond and its Application to Investigate the Effects of Ubiquitination and SUMOylation on α-Synuclein Aggregation and Toxicity.ACS chemical biology · 2016Article
- All about that Amide Bond: The Sixth Chemical Protein Synthesis (CPS) Meeting.Chembiochem : a European journal of chemical biology · 2015Article
- Nonspecific yet decisive: Ubiquitination can affect the native-state dynamics of the modified protein.Protein science : a publication of the Protein Society · 2015Article
- Curious Cases of the Enzymes.Journal of medical biochemistry · 2015Review
- Facile synthesis of native and protease-resistant ubiquitylated peptides.Chembiochem : a European journal of chemical biology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Ubiquitin (Ub) is a small 76 amino acid long protein that is highly conserved in all eukaryotes studied to date. In humans, more than 600 ligases are involved in the reversible modification of specific lysine side-chain amines in substrate proteins by conjugation with the C-terminal carboxylate of Ub. Initially monoubiquitylated proteins can undergo repetitive ubiquitylation starting from one of seven lysine residues or the α-amine in the first Ub to generate a variety of polyUb chains with different topologies and functions. The most well known role for protein ubiquitylation is in targeting substrates for proteolytic destruction by 26S proteasomes. However, a growing body of evidence indicates that both mono- and polyubiquitylation play proteasome-independent roles in modulating the structure, function, and localization of protein substrates. Understanding the complexity of Ub-mediated functions in our cells is a major challenge for modern biology. In addition to well-established in vivo genetic methods, biochemical and biophysical investigations of ubiquitylated proteins in vitro can shed light on the direct mechanistic roles for Ub in different contexts. Such studies have traditionally been limited by the ability to obtain sufficient quantities of homogenously ubiquitylated proteins with precisely defined linkages. This review focuses on recent advances in both synthetic and recombinant protein-based methods that have yielded access to homogenously site-specifically ubiquitylated proteins. Mechanistic studies of the roles for protein ubiquitylation and of the enzymes involved in protein deubiquitylation that are enabled by these chemical tools are highlighted.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.