ArticleThe Journal of membrane biology2013
Flow process for electroextraction of total proteins from microalgae.
Article in The Journal of membrane biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 100 citations in OpenAlex.
- Biphasic lipid extraction from microalgae after PEF-treatment reduces the energy demand of the downstream process.Biotechnology for biofuels and bioproducts · 2025Article
- Microalgae-Sustainable Source for Alternative Proteins and Functional Ingredients Promoting Gut and Liver Health.Global challenges (Hoboken, NJ) · 2023Review
- Recovering Microalgal Bioresources: A Review of Cell Disruption Methods and Extraction Technologies.Molecules (Basel, Switzerland) · 2022Review
- Application of Pulsed Electric Fields and High-Pressure Homogenization in Biorefinery Cascade ofFoods (Basel, Switzerland) · 2022Article
- Aquaculture Production of the Brown SeaweedsMolecules (Basel, Switzerland) · 2021Review
- Electroporation as a Solvent-Free Green Technique for Non-Destructive Extraction of Proteins and Lipids FromFrontiers in bioengineering and biotechnology · 2020Article
- Algal Proteins: Extraction, Application, and Challenges Concerning Production.Foods (Basel, Switzerland) · 2017Review
- A new mechanism for efficient hydrocarbon electro-extraction fromBiotechnology for biofuels · 2017Article
- Energy-efficient biomass processing with pulsed electric fields for bioeconomy and sustainable development.Biotechnology for biofuels · 2016Review
- A Comparative Study on the Effects of Millisecond- and Microsecond-Pulsed Electric Field Treatments on the Permeabilization and Extraction of Pigments from Chlorella vulgaris.The Journal of membrane biology · 2015Article
- Protein Extraction by Means of Electroporation from E. coli with Preserved Viability.The Journal of membrane biology · 2015Article
- Diatom milking: a review and new approaches.Marine drugs · 2015Review
- Electroporation in food processing and biorefinery.The Journal of membrane biology · 2014Review
- Effect of pulsed electric field treatments on permeabilization and extraction of pigments from Chlorella vulgaris.The Journal of membrane biology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Classical methods for protein extraction from microorganisms, used for large-scale treatments such as mechanical or chemical processes, affect the integrity of extracted cytosolic protein by releasing proteases contained in vacuoles. Our previous experiments on flow-process yeast electroextraction proved that pulsed electric field technology allows us to preserve the integrity of released cytosolic proteins by keeping intact vacuole membranes. Furthermore, large volumes are easily treated by the flow technology. Based on this previous knowledge, we developed a new protocol in order to electroextract total cytoplasmic proteins from microalgae (Nannochloropsis salina and Chlorella vulgaris). Given that induction of electropermeabilization is under the control of the target cell size, as the mean diameter for N. salina is only 2.5 μm, we used repetitive 2-ms-long pulses of alternating polarities with stronger field strengths than previously described for yeasts. The electric treatment was followed by a 24-h incubation period in a salty buffer. The amount of total protein released was evaluated by a classical Bradford assay. A more accurate evaluation of protein release was obtained by SDS-PAGE. Similar results were obtained with C. vulgaris under milder electrical conditions, as expected from their larger size. This innovative technology designed in our group should become familiar in the field of microalgae biotechnology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.