ArticleBMC genomics2013
Molecular cloning of doublesex genes of four cladocera (water flea) species.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 75 citations in OpenAlex.
- Evolution of environmental sex determination via juvenile hormone-induced gene co-option inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Sexual dimorphism in the tardigrade Paramacrobiotus metropolitanus transcriptome.Zoological letters · 2024Article
- The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn.iScience · 2023Article
- Evolution of sex determination in crustaceans.Marine life science & technology · 2023Review
- Effects of Functional Depletion ofInsects · 2021Article
- Evolutionary transition of doublesex regulation from sex-specific splicing to male-specific transcription in termites.Scientific reports · 2021Article
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- Full Functional Sex Reversal Achieved Through Silencing ofFrontiers in endocrinology · 2021Article
- A Crab Is Not a Fish: Unique Aspects of the Crustacean Endocrine System and Considerations for Endocrine Toxicology.Frontiers in endocrinology · 2021Review
- Independent evolution for sex determination and differentiation in theBiology open · 2019Article
- Phylogenetic analysis and embryonic expression of panarthropod Dmrt genes.Frontiers in zoology · 2019Article
- Doublesex Evolution Is Correlated with Social Complexity in Ants.Genome biology and evolution · 2018Article
- CrusTF: a comprehensive resource of transcriptomes for evolutionary and functional studies of crustacean transcription factors.BMC genomics · 2017Article
- De Novo Transcriptome Assembly and Sex-Biased Gene Expression in the Cyclical Parthenogenetic Daphnia galeata.Genome biology and evolution · 2016Article
- Metabolomics reveals an involvement of pantothenate for male production responding to the short-day stimulus in the water flea, Daphnia pulex.Scientific reports · 2016Article
- Article
- NMDA receptor activation upstream of methyl farnesoate signaling for short day-induced male offspring production in the water flea, Daphnia pulex.BMC genomics · 2015Article
- Expression analysis of Drosophila doublesex, transformer-2, intersex, fruitless-like, and vitellogenin homologs in the parahaploid predator Metaseiulus occidentalis (Chelicerata: Acari: Phytoseiidae).Experimental & applied acarology · 2015Article
- Identification and expression analysis of a doublesex1 gene in Daphnia pulex during different reproductive stages.Development genes and evolution · 2014Article
- Pan-metazoan phylogeny of the DMRT gene family: a framework for functional studies.Development genes and evolution · 2014Article
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Authors and funding
16 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe gene doublesex (dsx) is known as a key factor regulating genetic sex determination in many organisms. We previously identified two dsx genes (DapmaDsx1 and DapmaDsx2) from a freshwater branchiopod crustacean, Daphnia magna, which are expressed in males but not in females. D. magna produces males by parthenogenesis in response to environmental cues (environmental sex determination) and we showed that DapmaDsx1 expression during embryonic stages is responsible for the male trait development. The D. magna dsx genes are thought to have arisen by a cladoceran-specific duplication; therefore, to investigate evolutionary conservation of sex specific expression of dsx genes and to further assess their functions in the environmental sex determination, we searched for dsx homologs in four closely related cladoceran species.
resultsWe identified homologs of both dsx genes from, D. pulex, D. galeata, and Ceriodaphnia dubia, yet only a single dsx gene was found from Moina macrocopa. The deduced amino acid sequences of all 9 dsx homologs contained the DM and oligomerization domains, which are characteristic for all arthropod DSX family members. Molecular phylogenetic analysis suggested that the dsx gene duplication likely occurred prior to the divergence of these cladoceran species, because that of the giant tiger prawn Penaeus monodon is rooted ancestrally to both DSX1 and DSX2 of cladocerans. Therefore, this result also suggested that M. macrocopa lost dsx2 gene secondarily. Furthermore, all dsx genes identified in this study showed male-biased expression levels, yet only half of the putative 5' upstream regulatory elements are preserved in D. magna and D. pulex.
conclusionsThe all dsx genes of five cladoceran species examined had similar amino acid structure containing highly conserved DM and oligomerization domains, and exhibited sexually dimorphic expression patterns, suggesting that these genes may have similar functions for environmental sex determination in cladocerans.
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