ArticleThe Plant journal : for cell and molecular biology2013
Glutamate signalling via a MEKK1 kinase-dependent pathway induces changes in Arabidopsis root architecture.
Article in The Plant journal : for cell and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 71 citations in OpenAlex.
- Nitrate activates an MKK3-dependent MAPK module via NLP transcription factors in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- Why did glutamate, GABA, and melatonin become intercellular signalling molecules in plants?eLife · 2023Review
- Increased Ammonium Enhances Suboptimal-Temperature Tolerance in Cucumber Seedlings.Plants (Basel, Switzerland) · 2023Article
- MAPKKKs in Plants: Multidimensional Regulators of Plant Growth and Stress Responses.International journal of molecular sciences · 2023Review
- Poly-γ-glutamic acid promoted maize root development by affecting auxin signaling pathway and the abundance and diversity of rhizosphere microbial community.BMC plant biology · 2022Article
- Measuring Electrical Responses during Acute Exposure of Roots and Rhizoids of Plants to Compounds Using a Flow-Through System.Methods and protocols · 2022Article
- The Nitrate Transporter MtNPF6.8 Is a Master Sensor of Nitrate Signal in the Primary Root Tip ofFrontiers in plant science · 2022Article
- Article
- Proline-Mediated Drought Tolerance in the Barley (Plants (Basel, Switzerland) · 2021Article
- Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore Embryogenesis.Plants (Basel, Switzerland) · 2021Article
- Nitrogen Uptake in Plants: The Plasma Membrane Root Transport Systems from a Physiological and Proteomic Perspective.Plants (Basel, Switzerland) · 2021Review
- Receptor-Like Protein Kinases Function Upstream of MAPKs in Regulating Plant Development.International journal of molecular sciences · 2020Review
- Signaling pathways underlying nitrogen-dependent changes in root system architecture: from model to crop species.Journal of experimental botany · 2020Review
- Transcriptome Sequencing and iTRAQ of Different Rice Cultivars Provide Insight into Molecular Mechanisms of Cold-Tolerance Response in Japonica Rice.Rice (New York, N.Y.) · 2020Article
- Review
- Identification of the MAPK Cascade and its Relationship with Nitrogen Metabolism in the Green AlgaInternational journal of molecular sciences · 2020Article
- Mitogen activated protein kinase 6 and MAP kinase phosphatase 1 are involved in the response of Arabidopsis roots to L-glutamate.Plant molecular biology · 2018Article
- Identification of early ammonium nitrate-responsive genes in rice roots.Scientific reports · 2017Article
- QTL analysis of the developmental response to L-glutamate in Arabidopsis roots and its genotype-by-environment interactions.Journal of experimental botany · 2017Article
- Plant Chemical Genetics: From Phenotype-Based Screens to Synthetic Biology.Plant physiology · 2017Review
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
A chemical genetic approach has been used to investigate the mechanism by which external glutamate (l-Glu) is able to trigger major changes in root architecture in Arabidopsis thaliana L. An initial screen of 80 agonists and antagonists of mammalian glutamate and GABA receptors, using a specially developed 96-well microphenotyping system, found none that replicated the response of the root to l-Glu or antagonized it. However, a larger screen using >1500 molecules bioactive in Saccharomyces cerevisiae (yeast) identified two groups that interfered with the l-Glu response. One of the antagonists, 2-(4-chloro-3-methylphenyl)-2-oxoethyl thiocyanate (CMOT), has been reported to target Ste11, an evolutionarily conserved MAP kinase kinase kinase (MAP3K) in yeast. This led to the discovery that root growth in a triple mekk1 mekk2 mekk3 mutant (mekk1/2/3), defective in a set of three tandemly arranged MAP3Ks, was almost insensitive to l-Glu. However, the sensitivity of mekk1/2/3 roots to inhibition by other amino acids reported to act as agonists of glutamate receptor-like (GLR) channels in Arabidopsis roots (Asn, Cys, Gly and Ser) was unaffected. The l-Glu sensitivity of the mekk1/2/3 mutant was restored by transformation with a construct carrying the intact MEKK1 gene. These results demonstrate that MEKK1 plays a key role in transducing the l-Glu signal that elicits large-scale changes in root architecture, and provide genetic evidence for the existence in plants of an l-Glu signalling pathway analogous to that found in animals.
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