ArticleInternational journal of clinical and experimental pathology2013
Identification of CD68(+) neutrophil granulocytes in in vitro model of acute inflammation and inflammatory bowel disease.
Article in International journal of clinical and experimental pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
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- Defining and targeting macrophage heterogeneity in the mammary gland and breast cancer.Cancer medicine · 2024Review
- Molecular mapping of a core transcriptional signature of microglia-specific genes in schizophrenia.Translational psychiatry · 2023Article
- Association between spatial distribution of leukocyte subsets and clinical presentation of head and neck squamous cell carcinoma.Frontiers in immunology · 2023Article
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- Plasma cell-free RNA characteristics in COVID-19 patients.Genome research · 2022Article
- Effect of Yellow Propolis on Biocompatibility of Cements: Morphological and Immunohistochemistry Analysis.European journal of dentistry · 2022Article
- Scavenger receptors in host defense: from functional aspects to mode of action.Cell communication and signaling : CCS · 2022Review
- Neutrophil phenotypes implicated in the pathophysiology of post-traumatic sepsis.Frontiers in medicine · 2022Article
- A Human CD68 Promoter-Driven Inducible Cre-Recombinase Mouse Line Allows Specific Targeting of Tissue Resident Macrophages.Frontiers in immunology · 2022Article
- Macrophages in Atherosclerosis, First or Second Row Players?Biomedicines · 2021Review
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- Upregulation of microRNA-219-5p relieves ulcerative colitis through balancing the differentiation of Treg/Th17 cells.European journal of gastroenterology & hepatology · 2020Article
- Mesenchymal Stem Cells Promote the Resolution of Cardiac Inflammation After Ischemia Reperfusion Via Enhancing Efferocytosis of Neutrophils.Journal of the American Heart Association · 2020Article
- Noncanonical Mechanisms for Direct Bone Marrow Generating Ang II (Angiotensin II) Predominate in CD68 Positive Myeloid Lineage Cells.Hypertension (Dallas, Tex. : 1979) · 2020Article
- Expression of Leukocytes Following Myocardial Infarction in Rats is Modulated by Moderate White Wine Consumption.Nutrients · 2019Article
- Development of a 2-dimensional atlas of the human kidney with imaging mass cytometry.JCI insight · 2019Article
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- CD68/macrosialin: not just a histochemical marker.Laboratory investigation; a journal of technical methods and pathology · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
CD-68 is widely regarded as a selective marker for human monocytes and macrophages and is commonly used in human pathology studies. The purpose of this study was to investigate the expression of CD-68 in human peripheral blood mononuclear cells (PBMCs), neutrophil granulocytes (NGs) and in inflamed intestinal tissue samples for comparison. PBMCs and NGs were isolated from heparinized human blood samples. Intestinal biopsies were obtained during routine endoscopic procedures from patients with inflammatory bowel disease (IBD), e.g. ulcerative colitis and Crohn's disease. Gene and protein expression was analyzed by real-time RT-PCR, Western blot and immunohistochemistry. Both PBMCs and NGs preparations contained cells that were positive for CD-68 and either neutrophil elastase (NE), or myeloperoxidase (MPO). CD-68(+)/NE(-)/MPO(-) cells were regarded as monocytes. CD-68 mRNA expression was detected in PBMCs and NGs preparations. With Western blot and by performing immunoprecipitation of cell lysate, we could clearly detect CD-68 in NGs, U-937, THP-1, Hep-G2, Jurkat cells and PBMCs. Identification of inflammatory cells in acutely inflamed colonic mucosa obtained from patients with IBD revealed a strong accumulation of CD-68(+)/MPO(+) cells compared to normal colonic mucosa. The uptake of the marker by phagocytosis was excluded by performing a double staining with CD-163/NE and CD-163/MPO in PBMCs, NGs cultures and in inflamed colonic mucosa. These results identify CD-68(+) NGs in peripheral blood and inflamed colonic mucosa. CD-68 is not only a marker for the macrophages-monocytes but also for NGs.
Indexed as
Identifiers
23573303PMC3606846W2120512545What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.