ArticlePloS one2013
Volatile anesthetics, not intravenous anesthetic propofol bind to and attenuate the activation of platelet receptor integrin αIIbβ3.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Mechanism of isoflurane‑mediated breast cancer growthOncology letters · 2024Article
- Impact of general anesthesia on rotational thromboelastometry (ROTEM) parameters and standard plasmatic coagulation tests in healthy Beagle dogs.Veterinary and animal science · 2021Article
- The immunomodulatory mechanism of dexmedetomidine.International immunopharmacology · 2021Review
- Anesthetics isoflurane and sevoflurane attenuate flagellin-mediated inflammation in the lung.Biochemical and biophysical research communications · 2021Article
- Risk factors for pediatric surgical site infection following neurosurgical procedures for hydrocephalus: a retrospective single-center cohort study.BMC anesthesiology · 2021Article
- Surgical Site Infections and Perioperative Optimization of Host Immunity by Selection of Anesthetics.BioMed research international · 2021Review
- Mechanistic consideration of the effect of perioperative volatile anesthetics on phagocytes.Clinical immunology (Orlando, Fla.) · 2021Review
- The Role of Anesthetic Selection in Perioperative Bleeding.BioMed research international · 2021Review
- Effect of Isoflurane versus Propofol on the Early Outcome of Living Donor Adult Kidney Transplantation.International journal of organ transplantation medicine · 2021Article
- Volatile anesthetics affect macrophage phagocytosis.PloS one · 2019Article
- Pulmonary static inflation with 50% xenon attenuates decline in tissue factor in patients undergoing Stanford type A acute aortic dissection repair.Journal of thoracic disease · 2018Article
- Tirofiban, a Glycoprotein IIb/IIIa Antagonist, Has a Protective Effect on Decompression Sickness in Rats: Is the Crosstalk Between Platelet and Leukocytes Essential?Frontiers in physiology · 2018Article
- Statistical analysis plan for the WOMAN-ETAPlaT study: Effect of tranexamic acid on platelet function and thrombin generation.Wellcome open research · 2016Article
- Shedding Light on Anesthetic Mechanisms: Application of Photoaffinity Ligands.Anesthesia and analgesia · 2016Review
- Immune Modulation by Volatile Anesthetics.Anesthesiology · 2016Review
- Neurocognitive Adverse Effects of Anesthesia in Adults and Children: Gaps in Knowledge.Drug safety · 2016Review
- P-selectin-mediated platelet adhesion promotes tumor growth.Oncotarget · 2015Article
- Mechanisms revealed through general anesthetic photolabeling.Current anesthesiology reports · 2014Article
- Effect of Inhalation Anesthetics on Tumor Metastasis.Technology in cancer research & treatmentReview
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundIn clinical reports, the usage of isoflurane and sevoflurane was associated with more surgical field bleeding in endoscopic sinus surgeries as compared to propofol. The activation of platelet receptor αIIbβ3 is a crucial event for platelet aggregation and clot stability. Here we studied the effect of isoflurane, sevoflurane, and propofol on the activation of αIIbβ3.
methodsThe effect of anesthetics on the activation of αIIbβ3 was probed using the activation sensitive antibody PAC-1 in both cell-based (platelets and αIIbβ3 transfectants) and cell-free assays. The binding sites of isoflurane on αIIbβ3 were explored using photoactivatable isoflurane (azi-isoflurane). The functional implication of revealed isoflurane binding sites were studied using alanine-scanning mutagenesis.
resultsIsoflurane and sevoflurane diminished the binding of PAC-1 to wild-type αIIbβ3 transfectants, but not to the high-affinity mutant, β3-N305T. Both anesthetics also impaired PAC-1 binding in a cell-free assay. In contrast, propofol did not affect the activation of αIIbβ3. Residues adducted by azi-isoflurane were near the calcium binding site (an important regulatory site termed SyMBS) just outside of the ligand binding site. The mutagenesis experiments demonstrated that these adducted residues were important in regulating integrin activation.
conclusionsIsoflurane and sevoflurane, but not propofol, impaired the activation of αIIbβ3. Azi-isoflurane binds to the regulatory site of integrin αIIbβ3, thereby suggesting that isoflurane blocks ligand binding of αIIbβ3 in not a competitive, but an allosteric manner.
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