ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2012
Micropropagation of Pithecellobium dulce (Roxb.) Benth-a multipurpose leguminous tree and assessment of genetic fidelity of micropropagated plants using molecular markers.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- Enhanced wedelolactone content inFrontiers in plant science · 2023Article
- In vitro propagation from axillary buds of the endangered treePlant biotechnology (Tokyo, Japan) · 2021Article
- Alteration of media enables efficient in vitro cloning of maturePhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- In vitro propagation, ex vitro rooting and leaf micromorphology ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- RAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1.Meta gene · 2016Article
- Micropropagtion of Terminalia bellerica from nodal explants of mature tree and assessment of genetic fidelity using ISSR and RAPD markers.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Micropropagation of annatto (Bixa orellana L.) from mature tree and assessment of genetic fidelity of micropropagated plants with RAPD markers.Physiology and molecular biology of plants : an international journal of functional plant biology · 2013Article
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3 authors.
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Abstract
An efficient and reproducible protocol has been developed for in vitro propagation of Pithecellobium dulce (Roxb.) Benth (a multipurpose leguminous tree) from field grown nodal segments (axillary bud). Shoot bud induction occurred from nodal explants of 15-years-old tree on Murashige and Skoog (MS) basal medium supplemented with 4.4 μM 6-benzyladenine (BA) and multiplication was achieved on MS medium supplemented with 4.4 μM BA + 0.73 μM phenylacetic acid (PAA) i.e. up to 7 shoot buds in the period of 5-6 weeks. Addition of adenine sulphate (AdS) to this medium further enhanced the number of shoot buds up to 10. Proliferating shoot cultures were established by repeatedly subculturing primary culture on fresh medium (MS + 4.4 μM BA + 0.73 μM PAA) after every 25 days. In vitro rooting was achieved on MS medium supplemented with 2.46 μM Indole-3-butyric acid (IBA) + 41.63 μM activated charcoal (AC). The micropropagated shoots with well developed roots were acclimatized in green house in pots containing sand, soil and manure (1:1:1). Genetic stability of micropropagated clones was evaluated using Random amplified polymorphic DNA (RAPD) and Inter simple sequence repeat (ISSR) markers. The amplification products were monomorphic in micropropagated plants and similar to those of mother plant. No polymorphism was detected revealing the genetic uniformity of micropropagated plants. This is the first report of an efficient protocol for regeneration of P. dulce through organogenesis, which can be used for further genetic transformation and pharmaceutical purposes.
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