ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2010
Agrobacterium-mediated transformation in chickpea (Cicer arietinum L.) with an insecticidal protein gene: optimisation of different factors.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 37 citations in OpenAlex.
- Activity of Arabidopsis Rubisco small subunit promoter in various tissues of chickpea.3 Biotech · 2023Article
- Recalcitrance to transformation, a hindrance for genome editing of legumes.Frontiers in genome editing · 2023Review
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Chickpea (Plants (Basel, Switzerland) · 2022Review
- Genetic transformation of legumes: an update.Plant cell reports · 2021Review
- Article
- Efficient genetic transformation and CRISPR/Cas9-mediated genome editing in Lemna aequinoctialis.Plant biotechnology journal · 2019Article
- An Improved Transformation System for Cowpea (Frontiers in plant science · 2019Article
- Genotype-independentPlant methods · 2018Article
- Finger on the Pulse: Pumping Iron into Chickpea.Frontiers in plant science · 2017Review
- Agrobacterium-mediated in planta genetic transformation of sugarcane setts.Plant cell reports · 2015Article
- Improved Agrobacterium-mediated transformation of cowpea via sonication and vacuum infiltration.Plant cell reports · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium-mediated transformation in chickpea was developed using strain LBA4404 carrying nptII, uidA and cryIAc genes and transformants selected on Murashige and Skoog's basal medium supplemented with benzyladenine, kinetin and kanamycin. Integration of transgenes was demonstrated using polymerase chain reaction and Southern blot hybridization of T0 plants. The expression of CryIAc delta endotoxin and GUS enzyme was shown by enzyme linked immunosorbent assay and histochemical assay respectively. The transgenic plants (T0) showed more tolerance to infection by Helicoverpa armigera compared to control plants. Various factors such as explant source, cultivar type, different preculture treatment period of explants, co-cultivation period, acetosyringone supplementation, Agrobacterium harboring different plasmids, vacuum infiltration and sonication treatment were tested to study the influence on transformation frequency. The results indicated that use of epicotyl as explant, cultivar ICCC37, Agrobacterium harboring plasmid pHS102 as vector, preculture of explant for 48 h, co-cultivation period of 2 days at 25°C and vacuum infiltration for 15 min produced the best transformation results. Sonication treatment of explants with Agrobacteria for 80 s was found to increase the frequency of transformation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.