ArticleScientific reports2013
Single molecule unfolding and stretching of protein domains inside a solid-state nanopore by electric field.
Article in Scientific reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed.
- Label-Free Profiling of Immunoglobulin Isotypes Using Solid-State Nanopores.Small methods · 2026Article
- Heterogeneous Multilayer Nanopores via Chemically Tuned Dielectric Breakdown for Single-Molecule Sensing.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Hybrid Microtubule-Solid-State Nanopores for Single-Molecule Analysis.Electrophoresis · 2026Article
- Electroosmotic Flow-Driven Nanopore Translocation of Large, Conformationally Dynamic Proteins: Overcoming Steric and Electrostatic Barriers.Journal of the American Chemical Society · 2026Article
- Review
- Threading of Single Protein Molecules Through a Solid-State Nanopore.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Computational and experimental study of AC measurements performed by a double-nanohole plasmonic nanopore sensor on 20 nm silica nanoparticles.Sensing and Bio-Sensing Research · 2024Article
- Solid-State Nanopores for Biomolecular Analysis and Detection.Advances in biochemical engineering/biotechnology · 2024Review
- Multi-physics simulations and experimental comparisons for the optical and electrical forces acting on a silica nanoparticle trapped by a double-nanohole plasmonic nanopore sensor.Sensing and Bio-Sensing Research · 2023Article
- Unlocking the Power of Nanopores: Recent Advances in Biosensing Applications and Analog Front-End.Biosensors · 2023Review
- Nanopore sensors for single molecular protein detection: Research progress based on computer simulations.IET nanobiotechnology · 2023Review
- A simple method to make, trap and deform a vesicle in a gel.Scientific reports · 2023Article
- Use of a solid-state nanopore for profiling the transferrin receptor protein and distinguishing between transferrin receptor and its ligand protein.Electrophoresis · 2023Article
- Legume Seed Protein Digestibility as Influenced by Traditional and Emerging Physical Processing Technologies.Foods (Basel, Switzerland) · 2022Review
- A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices.Micromachines · 2022Review
- Thermal treatment alternatives for enzymes inactivation in fruit juices: Recent breakthroughs and advancements.Ultrasonics sonochemistry · 2022Review
- Distinguishing between Similar Miniproteins with Single-Molecule Nanopore Sensing: A Computational Study.ACS nanoscience Au · 2022Article
- Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts.Scientific reports · 2022Article
- Cataloguing the proteome: Current developments in single-molecule protein sequencing.Biophysics reviews · 2022Review
- Zero-mode waveguides and nanopore-based sequencing technologies accelerate single-molecule studies.Biophysics and physicobiology · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single molecule methods have provided a significantly new look at the behavior of biomolecules in both equilibrium and non-equilibrium conditions. Most notable are the stretching experiments performed by atomic force microscopes and laser tweezers. Here we present an alternative single molecule method that can unfold a protein domain, observed at electric fields greater than 10(6) V/m, and is fully controllable by the application of increasing voltages across the membrane of the pore. Furthermore this unfolding mechanism is characterized by measuring both the residence time of the protein within the nanopore and the current blockade. The unfolding data supports a gradual unfolding mechanism rather than the cooperative transition observed by classical urea denaturation experiments. Lastly it is shown that the voltage-mediated unfolding is a function of the stability of the protein by comparing two mutationally destabilized variants of the protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.