ArticleThe EMBO journal2013
Distinct roles for Sir2 and RNAi in centromeric heterochromatin nucleation, spreading and maintenance.
Article in The EMBO journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 84 citations in OpenAlex.
- Phosphorylation of HP1/Swi6 relieves competition with Suv39/Clr4 on nucleosomes and enables H3K9 trimethyl spreading.Nucleic acids research · 2025Article
- Phosphorylation of HP1/Swi6 relieves competition with Suv39/Clr4 on nucleosomes and enables H3K9 trimethyl spreading.bioRxiv : the preprint server for biology · 2025Article
- Fission yeast Caprin protein is required for efficient heterochromatin establishment.PLoS genetics · 2025Article
- Finding new roles of classic biomolecular condensates in the nucleus: Lessons from fission yeast.Cell insight · 2024Review
- Heterochromatin repeat organization at an individual level: Rex1BD and the 14-3-3 protein coordinate to shape the epigenetic landscape within heterochromatin repeats.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- RNA quality control factors nucleate Clr4/SUV39H and trigger constitutive heterochromatin assembly.Cell · 2024Article
- Identification of an active RNAi pathway inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Mathematical model for the role of multiple pericentromeric repeats on heterochromatin assembly.PLoS computational biology · 2024Article
- Rex1BD and the 14-3-3 protein control heterochromatin organization at tandem repeats by linking RNAi and HDAC.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
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- Breakers and amplifiers in chromatin circuitry: acetylation and ubiquitination control the heterochromatin machinery.Current opinion in structural biology · 2021Review
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- Defining the consequences of endogenous genetic variation within a novel family of Schizosaccharomyces pombe heterochromatin nucleating sequences.G3 (Bethesda, Md.) · 2021Article
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- Rbm10 facilitates heterochromatin assembly via the Clr6 HDAC complex.Epigenetics & chromatin · 2021Article
- Article
- Mkt1 is required for RNAi-mediated silencing and establishment of heterochromatin in fission yeast.Nucleic acids research · 2020Article
- Transgenerational epigenetic inheritance: from phenomena to molecular mechanisms.Current opinion in neurobiology · 2019Review
- Review
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6 authors at 2 institutions in 1 country.
Funding
Abstract
Epigenetically regulated heterochromatin domains govern essential cellular activities. A key feature of heterochromatin domains is the presence of hypoacetylated nucleosomes, which are methylated on lysine 9 of histone H3 (H3K9me). Here, we investigate the requirements for establishment, spreading and maintenance of heterochromatin using fission yeast centromeres as a paradigm. We show that establishment of heterochromatin on centromeric repeats is initiated at modular 'nucleation sites' by RNA interference (RNAi), ensuring the mitotic stability of centromere-bearing minichromosomes. We demonstrate that the histone deacetylases Sir2 and Clr3 and the chromodomain protein Swi6(HP1) are required for H3K9me spreading from nucleation sites, thus allowing formation of extended heterochromatin domains. We discovered that RNAi and Sir2 along with Swi6(HP1) operate in two independent pathways to maintain heterochromatin. Finally, we demonstrate that tethering of Sir2 is pivotal to the maintenance of heterochromatin at an ectopic locus in the absence of RNAi. These analyses reveal that Sir2, together with RNAi, are sufficient to ensure heterochromatin integrity and provide evidence for sequential establishment, spreading and maintenance steps in the assembly of centromeric heterochromatin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.