ArticleSensors (Basel, Switzerland)2013
Microflow cytometers with integrated hydrodynamic focusing.
Article in Sensors (Basel, Switzerland), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 48 citations in OpenAlex.
- The potential of microfluidic cell analysis in CAR T cell therapy manufacturing.Frontiers in bioengineering and biotechnology · 2025Review
- Integration of a Dielectrophoretic Tapered Aluminum Microelectrode Array with a Flow Focusing Technique.Sensors (Basel, Switzerland) · 2021Article
- Dry Film Resist Laminated Microfluidic System for Electrical Impedance Measurements.Micromachines · 2021Article
- Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics.Lab on a chip · 2021Article
- Disease diagnostics using hydrodynamic flow focusing in microfluidic devices: Beyond flow cytometry.Biomedical engineering letters · 2020Review
- A Microflow Cytometer with a Rectangular Quasi-Flat-Top Laser Spot.Sensors (Basel, Switzerland) · 2016Article
- Using binary optical elements (BOEs) to generate rectangular spots for illumination in micro flow cytometer.Biomicrofluidics · 2016Article
- Optofluidic Device Based Microflow Cytometers for Particle/Cell Detection: A Review.Micromachines · 2016Review
- Imaging Cells in Flow Cytometer Using Spatial-Temporal Transformation.Scientific reports · 2015Article
- Micro flow cytometer with self-aligned 3D hydrodynamic focusing.Biomedical optics express · 2015Article
- Lab-on-chip cytometry based on magnetoresistive sensors for bacteria detection in milk.Sensors (Basel, Switzerland) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study demonstrates the suitability of microfluidic structures for high throughput blood cell analysis. The microfluidic chips exploit fully integrated hydrodynamic focusing based on two different concepts: Two-stage cascade focusing and spin focusing (vortex) principle. The sample--A suspension of micro particles or blood cells--is injected into a sheath fluid streaming at a substantially higher flow rate, which assures positioning of the particles in the center of the flow channel. Particle velocities of a few m/s are achieved as required for high throughput blood cell analysis. The stability of hydrodynamic particle positioning was evaluated by measuring the pulse heights distributions of fluorescence signals from calibration beads. Quantitative assessment based on coefficient of variation for the fluorescence intensity distributions resulted in a value of about 3% determined for the micro-device exploiting cascade hydrodynamic focusing. For the spin focusing approach similar values were achieved for sample flow rates being 1.5 times lower. Our results indicate that the performances of both variants of hydrodynamic focusing suit for blood cell differentiation and counting. The potential of the micro flow cytometer is demonstrated by detecting immunologically labeled CD3 positive and CD4 positive T-lymphocytes in blood.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.