ArticleMethods (San Diego, Calif.)2013
Activation induced morphological changes and integrin αIIbβ3 activity of living platelets.
Article in Methods (San Diego, Calif.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Effect of two-dimensional spatial confinement on platelet mechanics.Biophysical reports · 2026Article
- Applications of Nanopipettes in Scanning Ion Conductance Microscopy for High-Spatial-Resolution Topographic Imaging and Sensing in Single Cells.ACS measurement science au · 2026Review
- Biomechanical anticoagulation by spherical platelets in extracorporeal systems.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Platelets cause microvascular occlusion and delayed neurological deficits after subarachnoid hemorrhage in mice.Experimental & molecular medicine · 2026Article
- Amyloid Protein-Induced Remodeling of Morphometry and Nanomechanics in Human Platelets.Biomedicines · 2025Article
- 12/15-Lipooxygenase Inhibition Reduces Microvessel Constriction and Microthrombi After Subarachnoid Hemorrhage in Mice.Translational stroke research · 2025Article
- Volume Regulation and Nonosmotic Volume of Individual Human Platelets Quantified by High-Speed Scanning Ion Conductance Microscopy.Thrombosis and haemostasis · 2025Article
- 12/15-Lipooxygenase Inhibition Reduces Microvessel Constriction and Microthrombi after Subarachnoid Hemorrhage in Mice.Research square · 2024Article
- Platelets' Nanomechanics and Morphology in Neurodegenerative Pathologies.Biomedicines · 2022Article
- Structure of shear-induced platelet aggregated clot formed in an in vitro arterial thrombosis model.Blood advances · 2022Article
- Morphometric and Nanomechanical Features of Platelets from Women with Early Pregnancy Loss Provide New Evidence of the Impact of Inherited Thrombophilia.International journal of molecular sciences · 2021Article
- Platelet heterogeneity enhances blood clot volumetric contraction: An example of asynchrono-mechanical amplification.Biomaterials · 2021Article
- A predictive multiscale model for simulating flow-induced platelet activation: Correlating in silico results with in vitro results.Journal of biomechanics · 2021Article
- Atomic Force Microscopy Imaging in Turbid Liquids: A Promising Tool in Nanomedicine.Sensors (Basel, Switzerland) · 2020Article
- Thrombin-induced cytoskeleton dynamics in spread human platelets observed with fast scanning ion conductance microscopy.Scientific reports · 2017Article
- A phenomenological particle-based platelet model for simulating filopodia formation during early activation.International journal for numerical methods in biomedical engineering · 2015Article
- Nanoimaging methods for biomedicine.Methods (San Diego, Calif.) · 2013Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Platelets are essential in hemostasis. Upon activation they undergo a shape-change accompanied with receptor presentation. Atomic force microscopy (AFM) imaging and single molecule force spectroscopy (SMFS) were used as powerful tools for exploring morphological changes as well as receptor activities of platelets. Imaging time series was accomplished with and without fixation steps at the single platelet level. Hereby the response of mechanical stimulation of the platelet by the AFM cantilever tip was directly observed. We demonstrate that living and fixed platelets develop filopodia after a short activation time followed by their disappearance including cellular bleb formation. Thereafter a second filopodia formation (filopodia extrusion) was observed; those filopodia subsequently disappeared again, and finally platelets detached from the support due to cell death. We determined the influence of mechanical stress on the chronology of morphological changes of platelets and demonstrated shear force induced filopodia formation. Through recordings over several hours, topographical AFM images over the full platelet lifetime - from early activation up to apoptosis - are presented. SMFS measurements on living platelets allowed determining the activation state of the most prominent membrane receptor integrin αIIbβ3 at all different phases of activation. αIIbβ3 was fully activated, independent of the morphological state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.