ArticleCells, tissues, organs2013
Delivery of mesenchymal stem cells in chitosan/collagen microbeads for orthopedic tissue repair.
Article in Cells, tissues, organs, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 83 citations in OpenAlex.
- Synthesis, Characterization and Biological Properties of Type I Collagen-Chitosan Mixed Hydrogels: A Review.Gels (Basel, Switzerland) · 2023Review
- Preparation of Cell-Loaded Microbeads as Stable and Injectable Delivery Platforms for Tissue Engineering.Biomimetics (Basel, Switzerland) · 2023Article
- Synergistic effects of 3D chitosan-based hybrid scaffolds and mesenchymal stem cells in orthopaedic tissue engineering.IET nanobiotechnology · 2023Review
- Complexed Polymer Film-Forming Spray: An Optimal Delivery System for Secretome of Mesenchymal Stem Cell as Diabetic Wound Dressing?Pharmaceuticals (Basel, Switzerland) · 2022Article
- Mesenchymal Stem Cells-Hydrogel Microspheres System for Bone Regeneration in Calvarial Defects.Gels (Basel, Switzerland) · 2022Article
- Advanced Hydrogels as Exosome Delivery Systems for Osteogenic Differentiation of MSCs: Application in Bone Regeneration.International journal of molecular sciences · 2021Review
- Article
- Modular microcarrier technologies for cell-based bone regeneration.Journal of materials chemistry. B · 2020Review
- Biocompatible Gels of Chitosan-Buriti Oil for Potential Wound Healing Applications.Materials (Basel, Switzerland) · 2020Article
- Injectable Cardiac Cell Microdroplets for Tissue Regeneration.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Hydrogel microparticles for biomedical applications.Nature reviews. Materials · 2020Article
- Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies.Stem cell research & therapy · 2019Review
- Engineered microscale hydrogels for drug delivery, cell therapy, and sequencing.Biomedical microdevices · 2019Review
- Development of Modular, Dual-Perfused Osteochondral Constructs for Cartilage Repair.Tissue engineering. Part C, Methods · 2019Article
- Bioactive hydrogels for bone regeneration.Bioactive materials · 2018Review
- Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment.Pharmaceutics · 2018Article
- Distributed vasculogenesis from modular agarose-hydroxyapatite-fibrinogen microbeads.Acta biomaterialia · 2017Article
- Assessment of bone regeneration of a tissue-engineered bone complex using human dental pulp stem cells/poly(ε-caprolactone)-biphasic calcium phosphate scaffold constructs in rabbit calvarial defects.Journal of materials science. Materials in medicine · 2017Article
- The Diverse Roles of Hydrogel Mechanics in Injectable Stem Cell Transplantation.Current opinion in chemical engineering · 2017Article
- Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues.ACS biomaterials science & engineering · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Microencapsulation and delivery of stem cells in biomaterials is a promising approach to repairing damaged tissue in a minimally invasive manner. An appropriate biomaterial niche can protect the embedded cells from the challenging environment in the host tissue, while also directing stem cell differentiation toward the desired lineage. In this study, adult human mesenchymal stem cells (MSC) were embedded in hydrogel microbeads consisting of chitosan and type I collagen using an emulsification process. Glyoxal and β-glycerophosphate were used as chemical and physical crosslinkers to initiate copolymerization of the matrix materials. The average size and size distribution of the microbeads could be varied by controlling the emulsification conditions. Spheroidal microbeads ranging in diameter from 82 ± 19 to 290 ± 78 µm were produced. Viability staining showed that MSC survived the encapsulation process (>90% viability) and spread inside the matrix over a period of 9 days in culture. Induced osteogenic differentiation using medium supplements showed that MSC increased gene expression of osterix and osteocalcin over time in culture, and also deposited calcium mineral. Bone sialoprotein and type I collagen gene expression were not affected. Delivery of microbeads through standard needles at practically relevant flow rates did not adversely affect cell viability, and microbeads could also be easily molded into prescribed geometries for delivery. Such protein-based microbeads may have utility in orthopedic tissue regeneration by allowing minimally invasive delivery of progenitor cells in microenvironments that are both protective and instructive.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.