ArticleJournal of biological engineering2013
Early differentiation patterning of mouse embryonic stem cells in response to variations in alginate substrate stiffness.
Article in Journal of biological engineering, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Microfluidics and organ-on-a-chip technologies: A systematic review of the methods used to mimic bone marrow.PloS one · 2020Pooled it
- Airflow controlled droplet bioprinting enables high-throughput generation of iPSC-derived thymic organoids withBiofabrication · 2026Article
- E-cadherin negatively regulates hESCs endodermal differentiation under varied substrate stiffnesses.Cellular and molecular life sciences : CMLS · 2025Article
- Tight Spaces, Tighter Signals: Spatial Constraints as Drivers of Peripheral Myelination.Cells · 2025Article
- Spatial cell fate manipulation of human pluripotent stem cells by controlling the microenvironment using photocurable hydrogel.Development (Cambridge, England) · 2024Article
- A Critical Review on Classified Excipient Sodium-Alginate-Based Hydrogels: Modification, Characterization, and Application in Soft Tissue Engineering.Gels (Basel, Switzerland) · 2023Review
- Microfluidic Organ-on-A-chip: A Guide to Biomaterial Choice and Fabrication.International journal of molecular sciences · 2023Review
- StemBond hydrogels control the mechanical microenvironment for pluripotent stem cells.Nature communications · 2021Article
- A Review of Biomaterials and Scaffold Fabrication for Organ-on-a-Chip (OOAC) Systems.Bioengineering (Basel, Switzerland) · 2021Review
- Self-Assembling Polypeptide Hydrogels as a Platform to Recapitulate the Tumor Microenvironment.Cancers · 2021Article
- Microvascular Tissue Engineering-A Review.Biomedicines · 2021Review
- Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives.Methods in molecular biology (Clifton, N.J.) · 2021Article
- A Minireview of Microfluidic Scaffold Materials in Tissue Engineering.Frontiers in molecular biosciences · 2021Review
- Epigenetic Erasing and Pancreatic Differentiation of Dermal Fibroblasts into Insulin-Producing Cells are Boosted by the Use of Low-Stiffness Substrate.Stem cell reviews and reports · 2018Article
- Article
- Differentiation of Wharton's jelly mesenchymal stem cells into neurons in alginate scaffold.Neural regeneration research · 2015Article
- Systems level approach reveals the correlation of endoderm differentiation of mouse embryonic stem cells with specific microstructural cues of fibrin gels.Journal of the Royal Society, Interface · 2014Article
- Article
- Islet and stem cell encapsulation for clinical transplantation.The review of diabetic studies : RDS · 2014Review
- Behavior of a Spontaneously Arising Human Retinal Pigment Epithelial Cell Line Cultivated on Thin Alginate Film.Journal of ophthalmic & vision researchArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEmbryonic stem cells (ESCs) have been implicated to have tremendous impact in regenerative therapeutics of various diseases, including Type 1 Diabetes. Upon generation of functionally mature ESC derived islet-like cells, they need to be implanted into diabetic patients to restore the loss of islet activity. Encapsulation in alginate microcapsules is a promising route of implantation, which can protect the cells from the recipient's immune system. While there has been a significant investigation into islet encapsulation over the past decade, the feasibility of encapsulation and differentiation of ESCs has been less explored. Research over the past few years has identified the cellular mechanical microenvironment to play a central role in phenotype commitment of stem cells. Therefore it will be important to design the encapsulation material to be supportive to cellular functionality and maturation.
resultsThis work investigated the effect of stiffness of alginate substrate on initial differentiation and phenotype commitment of murine ESCs. ESCs grown on alginate substrates tuned to similar biomechanical properties of native pancreatic tissue elicited both an enhanced and incrementally responsive differentiation towards endodermal lineage traits.
conclusionsThe insight into these biophysical phenomena found in this study can be used along with other cues to enhance the differentiation of embryonic stem cells toward a specific lineage fate.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.