ArticleJournal of ovarian research2013
Small SSEA-4-positive cells from human ovarian cell cultures: related to embryonic stem cells and germinal lineage?
Article in Journal of ovarian research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- The Cancer Stem Cell Niche in Ovarian Cancer and Its Impact on Immune Surveillance.International journal of molecular sciences · 2021Review
- Generation of Artificial Gamete and Embryo From Stem Cells in Reproductive Medicine.Frontiers in bioengineering and biotechnology · 2020Review
- In Vitro Generation of Oocytes from Ovarian Stem Cells (OSCs): In Search of Major Evidence.International journal of molecular sciences · 2019Review
- Presence and role of stem cells in ovarian cancer.World journal of stem cells · 2019Review
- Stem cells survive oncotherapy & can regenerate non-functional gonads: A paradigm shift for oncofertility.The Indian journal of medical research · 2018Review
- Functional Testing of Primitive Oocyte-like Cells Developed in Ovarian Surface Epithelium Cell Culture from Small VSEL-like Stem Cells: Can They Be Fertilized One Day?Stem cell reviews and reports · 2018Article
- Further characterization of adult sheep ovarian stem cells and their involvement in neo-oogenesis and follicle assembly.Journal of ovarian research · 2018Article
- Stem cells and their potential clinical applications in psychiatric disorders.Progress in neuro-psychopharmacology & biological psychiatry · 2018Review
- Making gametes from alternate sources of stem cells: past, present and future.Reproductive biology and endocrinology : RB&E · 2017Review
- Silencing PRDM14 expression by an innovative RNAi therapy inhibits stemness, tumorigenicity, and metastasis of breast cancer.Oncotarget · 2017Article
- Putative stem cells and epithelial-mesenchymal transition revealed in sections of ovarian tumor in patients with serous ovarian carcinoma using immunohistochemistry for vimentin and pluripotency-related markers.Journal of ovarian research · 2017Article
- Underlying Mechanisms that Restore Spermatogenesis on Transplanting Healthy Niche Cells in Busulphan Treated Mouse Testis.Stem cell reviews and reports · 2016Article
- Adult human pancreas-derived cells expressing stage-specific embryonic antigen 4 differentiate into Sox9-expressing and Ngn3-expressing pancreatic ducts in vivo.Stem cell research & therapy · 2016Article
- Used protocols for isolation and propagation of ovarian stem cells, different cells with different traits.Journal of ovarian research · 2016Review
- Novel population of small tumour-initiating stem cells in the ovaries of women with borderline ovarian cancer.Scientific reports · 2016Article
- Small putative NANOG, SOX2, and SSEA-4-positive stem cells resembling very small embryonic-like stem cells in sections of ovarian tissue in patients with ovarian cancer.Journal of ovarian research · 2016Article
- Novel Action of FSH on Stem Cells in Adult Mammalian Ovary Induces Postnatal Oogenesis and Primordial Follicle Assembly.Stem cells international · 2016Review
- Postnatal oogenesis in humans: a review of recent findings.Stem cells and cloning : advances and applications · 2015Review
- Making gametes from pluripotent stem cells--a promising role for very small embryonic-like stem cells.Reproductive biology and endocrinology : RB&E · 2014Article
- A multivariate approach to the integration of multi-omics datasets.BMC bioinformatics · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt has already been found that very small embyronic-like stem cells (VSELs) are present in adult human tissues and organs. The aim of this study was to find if there exists any similar population of cells in cell cultures of reproductive tissues and embryonic stem cells, and if these cells have any relation to pluripotency and germinal lineage. METHODS AND
resultsHere we report that a population of small SSEA-4-positive cells with diameters of up to 4 μm was isolated by fluorescence-activated cell sorting (FACS) from the human ovarian cell cultures after enzymatic degradation of adult cortex tissues. These small cells - putative ovarian stem cells - were also observed during cell culturing of up to 6 months and more. In general, small putative ovarian stem cells, isolated by FACS, showed a relatively low gene expression profile when compared to human embryonic stem cells (hESCs) and human adult fibroblasts; this may reflect the quiescent state of these cells. In spite of that, small putative ovarian stem cells expressed several genes related to primordial germ cells (PGCs), pluripotency and germinal lineage, including VASA. The PGC-related gene PRDM1 was strongly expressed in small putative ovarian stem cells; in both hESCs and fibroblasts it was significantly down-regulated. In addition, putative ovarian stem cells expressed other PGC-related genes, such as PRDM14 and DPPA3. Most of the pluripotency and germinal lineage-related genes were up-regulated in hESCs (except VASA). When compared to fibroblasts, there were several pluripotency-related genes, which were up-regulated in small putative ovarian stem cells. Similar populations of small cells were also isolated by FACS from human testicular and hESC cultures.
conclusionsOur results confirm the potential embryonic-like character of small putative stem cells isolated from human adult ovaries and their possible relation to germinal lineage.
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