ArticleEpigenetics & chromatin2013
Endogenous mammalian histone H3.3 exhibits chromatin-related functions during development.
Article in Epigenetics & chromatin, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers, 1 of them a synthesis that pooled it.
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Who cites it
59 citing papers in PubMed, 1 synthesis or guideline pooled it, 95 citations in OpenAlex.
- Contribution of histone variants to aneuploidy: a cancer perspective.Frontiers in genetics · 2023Pooled it
- Exon-skipping antisense oligonucleotides for H3.3K27M-altered diffuse midline glioma therapy.Molecular therapy. Nucleic acids · 2026Article
- REV1 Loss Triggers a G2/M Cell-Cycle Arrest Through Dysregulation of Mitotic Regulators.Genes · 2025Article
- A dynamic histone-based chromatin regulatory toolkit underpins genome and developmental evolution in an invertebrate clade.Genome biology · 2025Article
- The Histone Variant H3.3 Is Required for Plant Growth and Fertility inInternational journal of molecular sciences · 2024Article
- Review
- Phosphoproteome analysis of the crosstalk between sumoylation and phosphorylation in mouse spermatocytes.Biochemical and biophysical research communications · 2023Article
- Knockout tales: the versatile roles of histone H3.3 in development and disease.Epigenetics & chromatin · 2023Review
- Histone Variants and Their Chaperones in Hematological Malignancies.HemaSphere · 2023Review
- Developmentally programmed histone H3 expression regulates cellular plasticity at the parental-to-early embryo transition.Science advances · 2023Article
- A knockout-first model of H3f3a gene targeting leads to developmental lethality.Genesis (New York, N.Y. : 2000) · 2023Article
- Polyadenylation of canonical histone H3.1 in carcinogenesis.Advances in pharmacology (San Diego, Calif.) · 2023Article
- Histone H3.3 deposition in seed is essential for the post-embryonic developmental competence in Arabidopsis.Nature communications · 2022Article
- Histone Chaperones as Cardinal Players in Development.Frontiers in cell and developmental biology · 2022Review
- Stem-loop binding protein and metal carcinogenesis.Seminars in cancer biology · 2021Review
- Histone variants in skeletal myogenesis.Epigenetics · 2021Review
- Cell Fate Decisions in the Wake of Histone H3 Deposition.Frontiers in cell and developmental biology · 2021Review
- Review
- Histone H3.3 beyond cancer: Germline mutations inScience advances · 2020Article
- Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundThe histone variant H3.3 plays key roles in regulating chromatin states and transcription. However, the role of endogenous H3.3 in mammalian cells and during development has been less thoroughly investigated. To address this gap, we report the production and phenotypic analysis of mice and cells with targeted disruption of the H3.3-encoding gene, H3f3b.
resultsH3f3b knockout (KO) mice exhibit a semilethal phenotype traceable at least in part to defective cell division and chromosome segregation. H3f3b KO cells have widespread ectopic CENP-A protein localization suggesting one possible mechanism for defective chromosome segregation. KO cells have abnormal karyotypes and cell cycle profiles as well. The transcriptome and euchromatin-related epigenome were moderately affected by loss of H3f3b in mouse embryonic fibroblasts (MEFs) with ontology most notably pointing to changes in chromatin regulatory and histone coding genes. Reduced numbers of H3f3b KO mice survive to maturity and almost all survivors from both sexes are infertile.
conclusionsTaken together, our studies suggest that endogenous mammalian histone H3.3 has important roles in regulating chromatin and chromosome functions that in turn are important for cell division, genome integrity, and development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.