ArticleInternational journal of circumpolar health2013
Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada.
Article in International journal of circumpolar health, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Comparison of Three Real-Time PCR Assays Targeting the SSU rRNA Gene, the COWP Gene and the DnaJ-Like Protein Gene for the Diagnosis ofPathogens (Basel, Switzerland) · 2021Article
- High-Throughput Multiparallel Enteropathogen Detection via Nano-Liter qPCR.Frontiers in cellular and infection microbiology · 2020Article
- Chemical and microbial characteristics of municipal drinking water supply systems in the Canadian Arctic.Environmental science and pollution research international · 2018Article
- Wastewater treatment and public health in Nunavut: a microbial risk assessment framework for the Canadian Arctic.Environmental science and pollution research international · 2018Review
- Disinfection and removal of human pathogenic bacteria in arctic waste stabilization ponds.Environmental science and pollution research international · 2018Article
- A comparison of the Allplex™ bacterial and viral assays to conventional methods for detection of gastroenteritis agents.BMC research notes · 2018Article
- The global problem of childhood diarrhoeal diseases: emerging strategies in prevention and management.Therapeutic advances in infectious disease · 2018Review
- Cryptosporidium hominis Is a Newly Recognized Pathogen in the Arctic Region of Nunavik, Canada: Molecular Characterization of an Outbreak.PLoS neglected tropical diseases · 2016Article
- Multiplex polymerase chain reaction tests for detection of pathogens associated with gastroenteritis.Clinics in laboratory medicine · 2015Review
- Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in diarrhoeic patients in the Qikiqtani Region, Nunavut, Canada.International journal of circumpolar health · 2015Article
- Recent advances in clinical parasitology diagnostics.Current infectious disease reports · 2014Article
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
- Municipal water quantities and health in Nunavut households: an exploratory case study in Coral Harbour, Nunavut, Canada.International journal of circumpolar health · 2014Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundLittle is known about the microbiology of diarrhoeal disease in Canada's Arctic regions. There are a number of limitations of conventional microbiology testing techniques for diarrhoeal pathogens, and these may be further compromised in the Arctic, given the often long distances for specimen transport.
objectiveTo develop a novel multiple-target nanolitre real-time reverse transcriptase (RT)-PCR platform to simultaneously test diarrhoeal specimens collected from residents of the Qikiqtani (Baffin Island) Region of Nunavut, Canada, for a wide range of bacterial, parasitic and viral agents. STUDY DESIGN/
methodsDiarrhoeal stool samples submitted for bacterial culture to Qikiqtani General Hospital in Nunavut over an 18-month period were tested with a multiple-target nanolitre real-time PCR panel for major diarrhoeal pathogens including 8 bacterial, 6 viral and 2 parasitic targets.
resultsAmong 86 stool specimens tested by PCR, a total of 50 pathogens were detected with 1 or more pathogens found in 40 (46.5%) stool specimens. The organisms detected comprised 17 Cryptosporidium spp., 5 Clostridium difficile with toxin B, 6 Campylobacter spp., 6 Salmonella spp., 4 astroviruses, 3 noroviruses, 1 rotavirus, 1 Shigella spp. and 1 Giardia spp. The frequency of detection by PCR and bacterial culture was similar for Salmonella spp., but discrepant for Campylobacter spp., as Campylobacter was detected by culture from only 1/86 specimens. Similarly, Cryptosporidium spp. was detected in multiple samples by PCR but was not detected by microscopy or enzyme immunoassay.
conclusionsCryptosporidium spp., Campylobacter spp. and Clostridium difficile may be relatively common but possibly under-recognised pathogens in this region. Further study is needed to determine the regional epidemiology and clinical significance of these organisms. This method appears to be a useful tool for gastrointestinal pathogen research and may also be helpful for clinical diagnostics and outbreak investigation in remote regions where the yield of routine testing may be compromised.
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