ArticleAsian Pacific journal of tropical biomedicine2012
Cytotoxic (A549) and antimicrobial effects of Methylobacterium sp. isolate (ERI-135) from Nilgiris forest soil, India.
Article in Asian Pacific journal of tropical biomedicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 40 citations in OpenAlex.
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- Investigating the Toxicity of Compounds Yielded byToxins · 2022Article
- Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand.Metabolites · 2021Article
- GenomeFrontiers in genetics · 2021Article
- Contact Effect of aAntibiotics (Basel, Switzerland) · 2020Article
- Antimicrobial and Antioxidant Properties of a Bacterial Endophyte,International journal of microbiology · 2020Article
- Symbiont-mediated chemical defense in the invasive ladybirdEcology and evolution · 2019Article
- Plant Microbiome Innovation: M-trophs.Industrial biotechnology (New Rochelle, N.Y.) · 2018Article
- Optimization of the Cytotoxic Activity of ThreeBioMed research international · 2018Article
- Antimicrobial, antioxidant, and cytotoxic properties of vasicine acetate synthesized from vasicine isolated from Adhatoda vasica L.BioMed research international · 2015Article
- Inter individual variations of the fish skin microbiota: host genetics basis of mutualism?PloS one · 2014Article
- Biochemical constituents of a wild strain of Schizophyllum commune isolated from Achanakmar-Amarkantak Biosphere Reserve (ABR), India.World journal of microbiology & biotechnology · 2013Article
- Statistical optimization and anticancer activity of a red pigment isolated from Streptomyces sp. PM4.Asian Pacific journal of tropical biomedicine · 2013Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
objectiveTo assess the antimicrobial and cytotoxic effects of Methylobacterium sp. isolated from soil sample of Doddabetta forest, Nilgiris, Western Ghats of Tamil Nadu.
methodsIsolation of Methylobacterium was performed from soils by serial dilution plate technique. The strain was grown in modified nutrient gulucose agar (MNGA) medium to study the morphology and biochemical characteristics. Methylobacterium sp. was screened for its antimicrobial activity against pathogenic bacteria and fungi. The strain was subjected to 16S rRNA analysis and was identified as Methylobacterium sp. The nucleotide sequence of the 16S rRNA gene of the isolate exhibited close similarity with other Methylobacterium sp. and has been submitted to Genbank. The antibacterial substances were extracted using chloroform and ethyl acetate from MNGA medium in which ERI-135 had grown for 5 d at 30 °C. Cytotoxic effect was also studied. GC-MS analysis was carried out. The antimicrobial activity was assessed using broth micro dilution technique.
resultsEthyl acetate extract showed activity against bacteria such as Bacillus subtilis, Klebsiella pneumoniae (K. pneumoniae), Pseudomonas aeruginosa, Salmonella typhimurium, Shigella flexneri, Enterobacter aerogenes, Staphylococcus aureu and Staphylococcus epidermidis (S. epidermidis) and fungi such as, Candida albicans and Trichophyton rubrum. The lowest minimum inhibitory concentrations were: 250 µg/mL against S. epidermidis and 250µg/mL against K. pneumonia. The isolate had the ability to produce enzymes such as protease. The exyract showed cytotoxic effect in human adenocarcinoma cancer cell line (A549). GC-MS analysis showed the presence of isovaleric acid (3.64%), 2-Methylbutanoic acid (5.03%), isobutyramide (5.05%), N,N-oimethylformamide-di-t-butylacetal (9.79%), benzeneacetamide (15.56%), octyl butyl phthalate (3.59%) and diisooctyl phthalate (5.79) in the extract.
conclusionsMethylobacterium sp. (ERI-135) showed promising antibacterial and cytotoxic activity. This is the first report in the antimicrobial and cytotoxic effect of Methylobacterium sp.
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