ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2013
Atomic force microscopy reveals regional variations in the micromechanical properties of the pericellular and extracellular matrices of the meniscus.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 81 citations in OpenAlex.
- Spatial Characterization of the Human Meniscus: Bridging Biomechanics and Biochemistry Using Atomic Force Microscopy Micro-Indentation and Mass Spectrometry Imaging.Small methods · 2026Article
- Molecular Mechanisms of Chondrocyte Hypertrophy Mediated by Physical Cues and Therapeutic Strategies in Osteoarthritis.International journal of molecular sciences · 2026Review
- Structure, Mechanics, and Mechanobiology of Fibrocartilage Pericellular Matrix Mediated by Type V Collagen.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- In Situ Loading and Time-Resolved Synchrotron-Based Phase Contrast Tomography for the Mechanical Investigation of Connective Knee Tissues: A Proof-of-Concept Study.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A method for analyzing AFM force mapping data obtained from soft tissue cryosections.Journal of biomechanics · 2024Article
- Adeno-Associated Virus-Delivered Fibroblast Growth Factor 18 Gene Therapy Promotes Cartilage Anabolism.Cartilage · 2023Article
- Rapid specialization and stiffening of the primitive matrix in developing articular cartilage and meniscus.Acta biomaterialia · 2023Article
- "Slow walk" mimetic tensile loading maintains human meniscus tissue resident progenitor cells homeostasis in photocrosslinked gelatin hydrogel.Bioactive materials · 2023Article
- Biomechanical properties of porcine meniscus as determined via AFM: Effect of region, compartment and anisotropy.PloS one · 2023Article
- Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.Progress in biophysics and molecular biology · 2022Review
- Nanomechanical Mapping of Hard Tissues by Atomic Force Microscopy: An Application to Cortical Bone.Materials (Basel, Switzerland) · 2022Article
- Nidogen: A matrix protein with potential roles in musculoskeletal tissue regeneration.Genes & diseases · 2022Review
- Mechanical properties of meniscal circumferential fibers using an inverse finite element analysis approach.Journal of the mechanical behavior of biomedical materials · 2022Article
- Multiscale Strain Transfer in Cartilage.Frontiers in cell and developmental biology · 2022Review
- Impact of perlecan, a core component of basement membrane, on regeneration of cartilaginous tissues.Acta biomaterialia · 2021Review
- Intrinsic and growth-mediated cell and matrix specialization during murine meniscus tissue assembly.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- The role of TGF-β2 in cartilage development and diseases.Bone & joint research · 2021Article
- Type V collagen regulates the structure and biomechanics of TMJ condylar cartilage: A fibrous-hyaline hybrid.Matrix biology : journal of the International Society for Matrix Biology · 2021Article
- Perlecan in Pericellular Mechanosensory Cell-Matrix Communication, Extracellular Matrix Stabilisation and Mechanoregulation of Load-Bearing Connective Tissues.International journal of molecular sciences · 2021Review
- Structure and mechanical properties of high-weight-bearing and low-weight-bearing areas of hip cartilage at the micro- and nano-levels.BMC musculoskeletal disorders · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Regional variations in the composition and architecture of the extracellular matrix (ECM) and pericellular matrix (PCM) of the knee meniscus play important roles in determining the local mechanical environment of meniscus cells. In this study, atomic force microscopy was used to spatially map the mechanical properties of matched ECM and perlecan-labeled PCM sites within the outer, middle, and inner porcine medial meniscus, and to evaluate the properties of the proximal surface of each region. The elastic modulus of the PCM was significantly higher in the outer region (151.4 ± 38.2 kPa) than the inner region (27.5 ± 8.8 kPa), and ECM moduli were consistently higher than region-matched PCM sites in both the outer (320.8 ± 92.5 kPa) and inner (66.1 ± 31.4 kPa) regions. These differences were associated with a higher proportion of aligned collagen fibers and lower glycosaminoglycan content in the outer region. Regional variations in the elastic moduli and some viscoelastic properties were observed on the proximal surface of the meniscus, with the inner region exhibiting the highest moduli overall. These results indicate that matrix architecture and composition play an important role in the regional micromechanical properties of the meniscus, suggesting that the local stress-strain environment of meniscal cells may vary significantly among the different regions.
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Registered trials
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