ArticleIranian biomedical journal2013
Homocysteine induces heme oxygenase-1 expression via transcription factor Nrf2 activation in HepG2 cell.
Article in Iranian biomedical journal, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- The Role of Organosulfur Compounds as Nrf2 Activators and Their Antioxidant Effects.Antioxidants (Basel, Switzerland) · 2022Review
- Oxidative Stress, Vascular Endothelium, and the Pathology of Neurodegeneration in Retina.Antioxidants (Basel, Switzerland) · 2022Review
- Article
- Effects of Nrf2-Keap1 signaling pathway on antioxidant defense system and oxidative damage in the clams Ruditapes philippinarum exposure to PAHs.Environmental science and pollution research international · 2021Article
- Role of hydrogen sulfide in endothelial dysfunction: Pathophysiology and therapeutic approaches.Journal of advanced research · 2021Review
- Nrf2 mediates the protective effects of homocysteine by increasing the levels of GSH content in HepG2 cells.Molecular medicine reports · 2017Article
- High levels of homocysteine downregulate apolipoprotein E expression via nuclear factor kappa B.World journal of biological chemistry · 2016Article
- Effects of that ATRA inhibits Nrf2-ARE pathway on glial cells activation after intracerebral hemorrhage.International journal of clinical and experimental pathology · 2015Article
- Homocysteine downregulates gene expression of heme oxygenase-1 in hepatocytes.Nutrition & metabolism · 2014Article
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Authors and funding
7 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundElevated level of plasma homocysteine has been related to various diseases. Patients with hyperhomocysteinemia can develop hepatic steatosis and fibrosis. We hypothesized that oxidative stress induced by homocysteine might play an important role in pathogenesis of liver injury. Also, the cellular response designed to combat oxidative stress is primarily controlled by the transcription factor Nrf2, a principal inducer of anti-oxidant and phase II-related genes.
methodsHepG2 cells were treated with homocysteine in different time periods. Glutathione content was measured by flowcytometry. Using electrophoretic mobility shift assay (EMSA) and Western-blotting, anti-oxidant response element (ARE)-binding activity of Nrf2 for heme ocygenase-1 (HO-1) was demonstrated. Real time RT-PCR and Western-blotting were performed to evaluate whether homocysteine was able to induce mRNA and protein expression of HO-1.
resultsThe role of Nrf2 in cellular response to homocysteine is substantiated by the following observations in HepG2 cells exposed to homocysteine (i) Western-blotting revealed that Nrf2 is strongly stabilized and became detectable in nuclear extracts. (ii) EMSA demonstrated increased binding of Nrf2 to oligomers containing HO-1 promoter-specific ARE-binding site. (iii) Real time RT-PCR and Western-blotting revealed increased mRNA and protein expression of inducible gene HO-1 after treatment with homocysteine.
conclusionData presented in the current study provide direct evidence that the immediate cellular response to oxidative stress provoked by homocysteine is orchestrated mainly by the Nrf2-ARE pathway. Therefore, induction of Nrf2-ARE-dependent expression of HO-1 could be a therapeutic option for hepatic cells damage induced by homocysteine.
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