ArticleInsect biochemistry and molecular biology2013
Gloverins of the silkworm Bombyx mori: structural and binding properties and activities.
Article in Insect biochemistry and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Identification and expression of a novel antimicrobial peptide Gloverin and its antibacterial effect against Staphylococcus aureus.BMC microbiology · 2025Article
- Diversity of Antimicrobial Peptides in Silkworm.Life (Basel, Switzerland) · 2023Review
- Characterization of Recombinant Antimicrobial Peptide BMGlv2 Heterologously Expressed inInternational journal of molecular sciences · 2022Article
- Effects of Local and Systemic Immune Challenges on the Expression of Selected Salivary Genes in the Malaria MosquitoPathogens (Basel, Switzerland) · 2021Article
- Article
- Proteomic analysis of the processes leading to Madurella mycetomatis grain formation in Galleria mellonella larvae.PLoS neglected tropical diseases · 2020Article
- Antimicrobial peptides fromRSC advances · 2019Review
- Repurposing of Glycine-Rich Proteins in Abiotic and Biotic Stresses in the Lone-Star Tick (Frontiers in physiology · 2019Article
- Antibacterial Mechanism of Gloverin2 from Silkworm,International journal of molecular sciences · 2018Article
- Review
- Analysis of the early cellular and humoral responses of Galleria mellonella larvae to infection by Candida albicans.Virulence · 2018Article
- Optimized expression of the antimicrobial protein Gloverin from Galleria mellonella using stably transformed Drosophila melanogaster S2 cells.Cytotechnology · 2017Article
- Mapping and recombination analysis of two moth colour mutations, Black moth and Wild wing spot, in the silkworm Bombyx mori.Heredity · 2016Article
- Genome-wide characterization and expression profiling of immune genes in the diamondback moth, Plutella xylostella (L.).Scientific reports · 2015Article
- Insect antimicrobial peptides and their applications.Applied microbiology and biotechnology · 2014Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Gloverins are basic, glycine-rich and heat-stable antibacterial proteins (∼14- kDa) in lepidopteran insects with activity against Escherichia coli, Gram-positive bacteria, fungi and a virus. Hyalophora gloveri gloverin adopts a random coil structure in aqueous solution but has α-helical structure in membrane-like environment, and it may interact with the lipid A moiety of lipopolysaccharide (LPS). Manduca sexta gloverin binds to the O-specific antigen and outer core carbohydrate of LPS. In the silkworm Bombyx mori, there are four gloverins with slightly acidic to neutral isoelectric points. In this study, we investigate structural and binding properties and activities of B. mori gloverins (BmGlvs), as well as correlations between structure, binding property and activity. Recombinant BmGlv1-4 were expressed in bacteria and purified. Circular dichroism (CD) spectra showed that all four BmGlvs mainly adopted random coli structure (>50%) in aqueous solution in regardless of pH, but contained α-helical structure in the presence of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), smooth and rough mutants (Ra, Rc and Re) of LPS and lipid A. Plate ELISA assay showed that BmGlvs at pH 5.0 bound to rough mutants of LPS and lipid A but not to smooth LPS. Antibacterial activity assay showed that positively charged BmGlvs (at pH 5.0) were active against E. coli mutant strains containing rough LPS but inactive against E. coli with smooth LPS. Our results suggest that binding to rough LPS is the prerequisite for the activity of BmGlvs against E. coli.
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