ArticleJournal of visualized experiments : JoVE2013
Cell co-culture patterning using aqueous two-phase systems.
Article in Journal of visualized experiments : JoVE, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed.
- Gelatin Methacrylate Macroporous Cell Scaffold Fabrication via One-Pot Aqueous Two-Phase Separation.Journal of biomedical materials research. Part B, Applied biomaterials · 2025Article
- Engineered Tools to Study Intercellular Communication.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Oil-mediated high-throughput generation and sorting of water-in-water droplets.Microsystems & nanoengineering · 2020Article
- Confinement of Suspension-Cultured Cells in Polyethylene Glycol/Polyethylene Oxide-Albumin Aqueous Two-Phase Systems.Frontiers in chemistry · 2019Article
- Engineering Dynamic Biointerfaces.Current opinion in chemical engineering · 2016Article
- Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system.Scientific reports · 2015Article
- Phytochemicals potently inhibit migration of metastatic breast cancer cells.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Robotic production of cancer cell spheroids with an aqueous two-phase system for drug testing.Journal of visualized experiments : JoVE · 2015Article
- Rapid Prototyping of Heterotypic Cell-Cell Contacts.Journal of materials chemistry. B · 2013Article
- Patterning bacterial communities on epithelial cells.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell patterning technologies that are fast, easy to use and affordable will be required for the future development of high throughput cell assays, platforms for studying cell-cell interactions and tissue engineered systems. This detailed protocol describes a method for generating co-cultures of cells using biocompatible solutions of dextran (DEX) and polyethylene glycol (PEG) that phase-separate when combined above threshold concentrations. Cells can be patterned in a variety of configurations using this method. Cell exclusion patterning can be performed by printing droplets of DEX on a substrate and covering them with a solution of PEG containing cells. The interfacial tension formed between the two polymer solutions causes cells to fall around the outside of the DEX droplet and form a circular clearing that can be used for migration assays. Cell islands can be patterned by dispensing a cell-rich DEX phase into a PEG solution or by covering the DEX droplet with a solution of PEG. Co-cultures can be formed directly by combining cell exclusion with DEX island patterning. These methods are compatible with a variety of liquid handling approaches, including manual micropipetting, and can be used with virtually any adherent cell type.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.