ArticleJournal of ovarian research2013
Follicular stage-dependent regulation of apoptosis and steroidogenesis by prohibitin in rat granulosa cells.
Article in Journal of ovarian research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Mitochondria in Ovarian Aging and Reproductive Longevity.Ageing research reviews · 2020Review
- Polycystic ovary syndrome: possible involvement of androgen-induced, chemerin-mediated ovarian recruitment of monocytes/macrophages.Biology of reproduction · 2018Article
- Regulation of androgen receptor signaling by ubiquitination during folliculogenesis and its possible dysregulation in polycystic ovarian syndrome.Scientific reports · 2017Article
- Prohibitin regulates the FSH signaling pathway in rat granulosa cell differentiation.Journal of molecular endocrinology · 2016Article
- Prohibitin( PHB) roles in granulosa cell physiology.Cell and tissue research · 2016Review
- High levels of testosterone inhibit ovarian follicle development by repressing the FSH signaling pathway.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2015Article
- Multifaceted role of prohibitin in cell survival and apoptosis.Apoptosis : an international journal on programmed cell death · 2015Review
- Chlorpromazine-Induced Hyperprolactinemia on Rat's Uterus.Iranian biomedical journal · 2015Article
- Prohibitins role in cellular survival through Ras-Raf-MEK-ERK pathway.Journal of cellular physiology · 2014Review
- Prohibitin is associated with antioxidative protection in hypoxia/reoxygenation-induced renal tubular epithelial cell injury.Scientific reports · 2013Article
- Cabergoline Suppresses FSH-Induced Differentiation and Steroidogenesis in Primary Cultured Rat Granulosa Cells.Reproductive medicine and biologyArticle
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFollicular growth and atresia are tightly regulated processes, which involve the participation of endocrine, autocrine and paracrine factors at the cellular level. Prohibitin (PHB) is a multifunctional intracellular protein playing an important role in the regulation of proliferation, apoptosis and differentiation. Here we examined the expression of PHB and its regulation by FSH in vitro and studied the role of PHB in the regulation of apoptosis and steroidogenesis in response to the apoptosis inducer staurosporine (STS) and to FSH, respectively.
methodsUndifferentiated and differentiated granulosa cells were collected from diethylstilbestrol (DES)- and equine chronic gonadotropin (eCG)-primed immature rats, respectively and then cultured with various treatments (FSH, adenovirus infection, STS) according to experimental design. The apoptosis rate, the production of estradiol and progesterone, and the expression of distinct proteins (PHB, caspase-3, phospho- and total Akt) were assessed.
resultsPHB is anti-apoptotic and its action is dependent on the differentiated state of the granulosa cells. Data from gain- and loss-of-function experiments demonstrate that PHB inhibited STS-induced caspase-3 cleavage and apoptosis in undifferentiated granulosa cells, but was ineffective in differentiated cells. In contrast, PHB suppresses FSH-induced steroidogenesis and this response is evident irrespective of the differentiated state of granulosa cells.
conclusionThese findings suggest that PHB regulates granulosa cell apoptosis and steroidogenesis in a follicular stage-dependent manner and that the dysregulation of PHB expression and action may be relevant to ovarian dysfunction.
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Registered trials
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