Evidence map›Paper›PMID 23566761›Full record

ArticleBioorganic & medicinal chemistry2013

Probing the stabilizing effects of modified nucleotides in the bacterial decoding region of 16S ribosomal RNA.

Santosh K Mahto, Christine S Chow

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioorganic & medicinal chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Santosh K MahtoDepartment of Chemistry, Wayne State University, Detroit, MI 48202, USA.
Christine S Chow

Funding

Anti-infectives that target bacterial ribosomesU01AI061192 · NIAID · WAYNE STATE UNIVERSITY · PI CUNNINGHAM, PHILIP R · 2004 to 2007
$2.5M
RIBOSOMAL FUNCTION &ANTIBIOTIC DESIGNR01AI055496 · NIAID · WAYNE STATE UNIVERSITY · PI MOBASHERY, SHAHRIAR · 2003 to 2007
$1.3M
The Role of Ribosomal RNA ModificationsR01GM087596 · NIGMS · WAYNE STATE UNIVERSITY · PI CHOW, CHRISTINE S · 2009 to 2012
$1.1M
NIAID NIH HHS AI055496NIAID NIH HHS AI061192NIAID NIH HHS R01 AI055496NIAID NIH HHS U01 AI061192NIGMS NIH HHS GM087596NIGMS NIH HHS R01 GM087596
6 · The paper itself

Abstract

The bacterial decoding region of 16S ribosomal RNA has multiple modified nucleotides. In order to study the role of N(4),2'-O-dimethylcytidine (m(4)Cm), the corresponding phosphoramidite was synthesized utilizing 5'-silyl-2'-ACE chemistry. Using solid-phase synthesis, m(4)Cm, 5-methylcytidine (m(5)C), 3-methyluridine (m(3)U), and 2'-O-methylcytidine (Cm) were site-specifically incorporated into small RNAs representing the decoding regions of different bacterial species. Biophysical studies were then used to provide insight into the stabilizing roles of the modified nucleotides. These studies reveal that methylation of cytidine and uridine has different effects. The same modifications at different positions or sequence contexts within similar RNA constructs also have contrasting roles, such as stabilizing or destabilizing the RNA helix.

Indexed as

Circular DichroismCytidineNucleotidesOrganophosphorus CompoundsRNA, BacterialRNA, Ribosomal, 16SUridine3-methyluridine5-methylcytidineCytidineNucleotidesOrganophosphorus CompoundsphosphoramiditeRNA, BacterialRNA, Ribosomal, 16SUridine

Identifiers

PMID23566761
PMCPMC3683315

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.