ArticleBMC genomics2013
Identification of genes related to agarwood formation: transcriptome analysis of healthy and wounded tissues of Aquilaria sinensis.
Article in BMC genomics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 160 citations in OpenAlex.
- Metabolites associated with endophytic fungi in agarwood: fungal compounds and host metabolites triggered by fungi.Mycology · 2026Review
- Molecular cloning, prokaryotic expression and induction characteristics of the sesquiterpene synthase gene (Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Optimization and induction effect evaluation of complex inducer of Aquilaria sinensis based on factorial design.Scientific reports · 2025Article
- Biosynthesis and Its Regulatory Mechanisms of 2-(2-Phenylethyl)chromones in Agarwood.Plants (Basel, Switzerland) · 2025Review
- De novo transcriptome assembly and analysis during agarwood induction in Gyrinops versteegii Gilg. seedling.Scientific reports · 2025Article
- Towards sustainable agarwood production: integrating microbial interactions, anatomical changes, and metabolite biosynthesis.Journal of industrial microbiology & biotechnology · 2024Review
- The Therapeutic Potential of Agarwood as an Antimicrobial and Anti-Inflammatory Agent: A Scoping Review.Antibiotics (Basel, Switzerland) · 2024Article
- Article
- Comparative Analysis of Metabolome and Transcriptome in Different Tissue Sites ofMolecules (Basel, Switzerland) · 2024Article
- 2-(2-Phenylethyl)chromones increase inFrontiers in plant science · 2024Article
- Research on using Aquilaria sinensis callus to evaluate the agarwood-inducing potential of fungi.PloS one · 2024Article
- Article
- Progressive Vertical and Horizontal Phytocompound Changes during Agarwood Formation inLife (Basel, Switzerland) · 2023Article
- Wound-Induced Temporal Reprogramming of Gene Expression during Agarwood Formation inPlants (Basel, Switzerland) · 2023Article
- Revealing the Roles of the JAZ Family in Defense Signaling and the Agarwood Formation Process inInternational journal of molecular sciences · 2023Article
- Article
- Identification and characterization of novel sesquiterpene synthases TPS9 and TPS12 fromPeerJ · 2023Article
- Genome-wide analysis of respiratory burst oxidase homolog (Frontiers in plant science · 2023Article
- Comparative transcriptome analysis reveals different defence responses during the early stage of wounding stress in Chi-Nan germplasm and ordinary Aquilaria sinensis.BMC plant biology · 2022Article
- Integrating Multiple Omics IdentifiesMicrobiology spectrum · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAgarwood is an expensive resinous heartwood derived from Aquilaria plants that is widely used in traditional medicines, incense and perfume. Only wounded trees can produce agarwood, and the huge demand for the agarwood products has led all Aquilaria spp. being endangered and listed in the Appendix II of the CITES (http://www.cites.org). The major components of agarwood are sesquiterpenes and phenylethyl chromones. Owing to a lack of genomic information, the molecular basis of wound-induced sesquiterpenes biosynthesis and agarwood formation remains unknown.
resultsTo identify the primary genes that maybe related to agarwood formation, we sequenced 2 cDNA libraries generated from healthy and wounded A. sinensis (Lour.) Gilg. A total of 89,137 unigenes with an average length of 678.65 bp were obtained, and they were annotated in detail at bioinformatics levels. Of those associated with agarwood formation, 30 putatively encoded enzymes in the sesquiterpene biosynthesis pathway, and a handful of transcription factors and protein kinases were related to wound signal transduction. Three full-length cDNAs of sesquiterpene synthases (ASS1-3) were cloned and expressed in Escherichia coli, and enzyme assays revealed that they are active enzymes, with the major products being δ-guaiene. A methyl jasmonate (MJ) induction experiment revealed that the expression of ASS was significantly induced by MJ, and the production of sesquiterpenes was elevated accordingly. The expression of some transcription factors and protein kinases, especially MYB4, WRKY4, MPKK2 and MAPK2, was also induced by MJ and coordinated with ASS expression, suggesting they maybe positive regulators of ASS.
conclusionsThis study provides extensive transcriptome information for Aquilaria spp. and valuable clues for elucidating the mechanism of wound-induced agarwood sesquiterpenes biosynthesis and their regulation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.